The spatially focused reagent injection system using 3-D sheath flow scanner integrated with SiO2 mesh strainer

Yasuo Suzuki; Masaki Kanai; Takahiro Nishimoto; Shuichi Shoji

The spatially focused reagent injection system using 3-D sheath flow scanner integrated with SiO₂ mesh strainer

Yasuo Suzuki, Masaki Kanai, Takahiro Nishimoto, Shuichi Shoji

School of Fundamental Science and Engineering

Research output: Contribution to conference › Paper › peer-review

Abstract

We developed a spatially focused reagent injection system with improved 3-D sheath flow scanner. SiO₂ mesh strainer is formed in the microwell to improve spatial resolution of the reagent flow. This enables selective delivery of the reagent to target cell colonies cultivated in the microwell. To fabricate the SiO₂ mesh strainer, sacrificial layer etching of Si micro pillars is employed. Simulation and experimental results confirmed that a use of the mesh strainer improves spatial resolution of the induced reagent successfully.

Original language	English
Pages	630-632
Number of pages	3
Publication status	Published - 2006 Jan 1
Event	10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 - Tokyo, Japan Duration: 2006 Nov 5 → 2006 Nov 9

Conference

Conference	10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006
Country/Territory	Japan
City	Tokyo
Period	06/11/5 → 06/11/9

Keywords

3-D sheath flow
Cell analysis
Micro mesh
Sample injection

ASJC Scopus subject areas

Chemical Engineering (miscellaneous)
Bioengineering

Cite this

The spatially focused reagent injection system using 3-D sheath flow scanner integrated with SiO₂ mesh strainer. / Suzuki, Yasuo; Kanai, Masaki; Nishimoto, Takahiro et al.
2006. 630-632 Paper presented at 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006, Tokyo, Japan.

Research output: Contribution to conference › Paper › peer-review

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author = "Yasuo Suzuki and Masaki Kanai and Takahiro Nishimoto and Shuichi Shoji",

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AU - Suzuki, Yasuo

AU - Kanai, Masaki

AU - Nishimoto, Takahiro

AU - Shoji, Shuichi

PY - 2006/1/1

Y1 - 2006/1/1

N2 - We developed a spatially focused reagent injection system with improved 3-D sheath flow scanner. SiO2 mesh strainer is formed in the microwell to improve spatial resolution of the reagent flow. This enables selective delivery of the reagent to target cell colonies cultivated in the microwell. To fabricate the SiO2 mesh strainer, sacrificial layer etching of Si micro pillars is employed. Simulation and experimental results confirmed that a use of the mesh strainer improves spatial resolution of the induced reagent successfully.

AB - We developed a spatially focused reagent injection system with improved 3-D sheath flow scanner. SiO2 mesh strainer is formed in the microwell to improve spatial resolution of the reagent flow. This enables selective delivery of the reagent to target cell colonies cultivated in the microwell. To fabricate the SiO2 mesh strainer, sacrificial layer etching of Si micro pillars is employed. Simulation and experimental results confirmed that a use of the mesh strainer improves spatial resolution of the induced reagent successfully.

KW - 3-D sheath flow

KW - Cell analysis

KW - Micro mesh

KW - Sample injection

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