TY - JOUR
T1 - The splice variant Ntr encoded by the tobacco resistance gene N has arole for negative regulation of antiviral defense responses
AU - Sasaki, Nobumitsu
AU - Takaoka, Masumi
AU - Sasaki, Shobu
AU - Hirai, Katsuyuki
AU - Meshi, Tetsuo
AU - Nyunoya, Hiroshi
N1 - Funding Information:
We thank Dr. Yasuhiko Matsushita for valuable suggestions and Dr. Mayumi Takano for technical assistance. This work was supported in part by a Grant-in-Aid for Scientific Research (to H.N. no. 22580046 ), Grants-in-Aid for Encouragement of Scientists (B) (to N.S. nos. 20780030 and 22580046 ) from the Ministry of Education, Culture, Sports, Science, and Technology of Japan.
PY - 2013/10
Y1 - 2013/10
N2 - Tobamovirus infection or transient elicitor expression induces a hypersensitive response (HR) in tobacco plants carrying the N resistance gene, which produces two gene products (N and Ntr) through alternative splicing. In this study, the biological significance of Ntr was investigated. The transient overexpression of Ntr markedly inhibited the induction of HR and N-dependent antiviral defense responses in tobacco and Nicotiana benthamiana, respectively. In addition, there were no drastic changes observed in the ratio of the two alternative transcripts during early tobamovirus infection. Our data suggest that Ntr may play a role as a negative regulator of N-dependent antiviral defenses.
AB - Tobamovirus infection or transient elicitor expression induces a hypersensitive response (HR) in tobacco plants carrying the N resistance gene, which produces two gene products (N and Ntr) through alternative splicing. In this study, the biological significance of Ntr was investigated. The transient overexpression of Ntr markedly inhibited the induction of HR and N-dependent antiviral defense responses in tobacco and Nicotiana benthamiana, respectively. In addition, there were no drastic changes observed in the ratio of the two alternative transcripts during early tobamovirus infection. Our data suggest that Ntr may play a role as a negative regulator of N-dependent antiviral defenses.
KW - Alternative splicing
KW - Antiviral defense responses
KW - Hypersensitive response
KW - N resistance gene
KW - Splice variant
KW - Tobamovirus
UR - http://www.scopus.com/inward/record.url?scp=84884386958&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84884386958&partnerID=8YFLogxK
U2 - 10.1016/j.pmpp.2013.08.002
DO - 10.1016/j.pmpp.2013.08.002
M3 - Article
AN - SCOPUS:84884386958
SN - 0885-5765
VL - 84
SP - 92
EP - 98
JO - Physiological and Molecular Plant Pathology
JF - Physiological and Molecular Plant Pathology
IS - 1
ER -