The yeast linear DNA killer plasmids, pGKL1 and pGKL2, possess terminally attached proteins

Yo Kikuchi; Keiko Hirai; Fumio Hishinuma

doi:10.1093/nar/12.14.5685

The yeast linear DNA killer plasmids, pGKL1 and pGKL2, possess terminally attached proteins

Yo Kikuchi^*, Keiko Hirai, Fumio Hishinuma

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

43 Citations (Scopus)

Abstract

The terminal structures of linear DNA killer plasmids from yeast, pGKL1 and pGKL2, were analyzed. Results obtained by exonuclease treatments of these plasmids show that both pGKL plasmids have free hydroxyl 3′-ends and blocked 5′-ends. Electrophoretic analysis of the terminal restriction fragments treated with proteases revealed that pGKL1 and pGKL2 have proteins bound at 5′termini and that the terminal protein of pGKL1 is distinct from that of pGKL2. This is the first linear DNA-terminal protein association found in yeast.

Original language	English
Pages (from-to)	5685-5692
Number of pages	8
Journal	Nucleic Acids Research
Volume	12
Issue number	14
DOIs	https://doi.org/10.1093/nar/12.14.5685
Publication status	Published - 1984 Jul 25
Externally published	Yes

ASJC Scopus subject areas

Statistics, Probability and Uncertainty
Applied Mathematics
Health, Toxicology and Mutagenesis
Toxicology
Genetics(clinical)
Genetics

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abstract = "The terminal structures of linear DNA killer plasmids from yeast, pGKL1 and pGKL2, were analyzed. Results obtained by exonuclease treatments of these plasmids show that both pGKL plasmids have free hydroxyl 3′-ends and blocked 5′-ends. Electrophoretic analysis of the terminal restriction fragments treated with proteases revealed that pGKL1 and pGKL2 have proteins bound at 5′termini and that the terminal protein of pGKL1 is distinct from that of pGKL2. This is the first linear DNA-terminal protein association found in yeast.",

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AU - Kikuchi, Yo

AU - Hirai, Keiko

AU - Hishinuma, Fumio

PY - 1984/7/25

Y1 - 1984/7/25

N2 - The terminal structures of linear DNA killer plasmids from yeast, pGKL1 and pGKL2, were analyzed. Results obtained by exonuclease treatments of these plasmids show that both pGKL plasmids have free hydroxyl 3′-ends and blocked 5′-ends. Electrophoretic analysis of the terminal restriction fragments treated with proteases revealed that pGKL1 and pGKL2 have proteins bound at 5′termini and that the terminal protein of pGKL1 is distinct from that of pGKL2. This is the first linear DNA-terminal protein association found in yeast.

AB - The terminal structures of linear DNA killer plasmids from yeast, pGKL1 and pGKL2, were analyzed. Results obtained by exonuclease treatments of these plasmids show that both pGKL plasmids have free hydroxyl 3′-ends and blocked 5′-ends. Electrophoretic analysis of the terminal restriction fragments treated with proteases revealed that pGKL1 and pGKL2 have proteins bound at 5′termini and that the terminal protein of pGKL1 is distinct from that of pGKL2. This is the first linear DNA-terminal protein association found in yeast.

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The yeast linear DNA killer plasmids, pGKL1 and pGKL2, possess terminally attached proteins

Abstract

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