TY - JOUR
T1 - Thrombopoietin induces production of nucleated thrombocytes from liver cells in Xenopus laevis
AU - Tanizaki, Yuta
AU - Ichisugi, Megumi
AU - Obuchi-Shimoji, Miyako
AU - Ishida-Iwata, Takako
AU - Tahara-Mogi, Ayaka
AU - Meguro-Ishikawa, Mizue
AU - Kato, Takashi
N1 - Funding Information:
We wish to thank Drs. Motoyasu Adachi, Taro Tamada and Ryota Kuroki at Quantum Beam Science Directorate, Japan Atomic Energy Agency, Japan for their support in preparing recombinant xlTPO. This work was supported in part by a Grant-in-Aid for Scientific Research from the Japan Society for the Promotion of Science and from Waseda University grants for special research projects. Part of this study was performed as a component of a Private University High-Tech Research Center project supported by the Japanese Ministry of Education, Culture, Sports, Science, and Technology and MEXT-Supported Program for the Strategic Research Foundation at Private Universities, 2008-2012. This work was supported in part by a grant from the Japan Society for the Promotion of Science (to Y. T.).
PY - 2015/12/21
Y1 - 2015/12/21
N2 - The development of mammalian megakaryocytes (MKs) and platelets, which are thought to be absent in non-mammals, is primarily regulated by the thrombopoietin (TPO)/Mpl system. Although non-mammals possess nucleated thrombocytes instead of platelets, the features of nucleated thrombocyte progenitors remain to be clarified. Here, we provide the general features of TPO using Xenopus laevis TPO (xlTPO). Hepatic and splenic cells were cultured in liquid suspension with recombinant xlTPO. These cells differentiated into large, round, polyploid CD41-expressing cells and were classified as X. laevis MKs, comparable to mammalian MKs. The subsequent culture of MKs after removal of xlTPO produced mature, spindle-shaped thrombocytes that were activated by thrombin, thereby altering their morphology. XlTPO induced MKs in cultured hepatic cells for at least three weeks; however, this was not observed in splenic cells; this result demonstrates the origin of early haematopoietic progenitors in the liver rather than the spleen. Additionally, xlTPO enhanced viability of peripheral thrombocytes, indicating the xlTPO-Mpl pathway stimulates anti-apoptotic in peripheral thrombocytes. The development of thrombocytes from MKs via the TPO-Mpl system in X. laevis plays a crucial role in their development from MKs, comparable to mammalian thrombopoiesis. Thus, our results offer insight into the cellular evolution of platelets/MKs in vertebrates. (200/200).
AB - The development of mammalian megakaryocytes (MKs) and platelets, which are thought to be absent in non-mammals, is primarily regulated by the thrombopoietin (TPO)/Mpl system. Although non-mammals possess nucleated thrombocytes instead of platelets, the features of nucleated thrombocyte progenitors remain to be clarified. Here, we provide the general features of TPO using Xenopus laevis TPO (xlTPO). Hepatic and splenic cells were cultured in liquid suspension with recombinant xlTPO. These cells differentiated into large, round, polyploid CD41-expressing cells and were classified as X. laevis MKs, comparable to mammalian MKs. The subsequent culture of MKs after removal of xlTPO produced mature, spindle-shaped thrombocytes that were activated by thrombin, thereby altering their morphology. XlTPO induced MKs in cultured hepatic cells for at least three weeks; however, this was not observed in splenic cells; this result demonstrates the origin of early haematopoietic progenitors in the liver rather than the spleen. Additionally, xlTPO enhanced viability of peripheral thrombocytes, indicating the xlTPO-Mpl pathway stimulates anti-apoptotic in peripheral thrombocytes. The development of thrombocytes from MKs via the TPO-Mpl system in X. laevis plays a crucial role in their development from MKs, comparable to mammalian thrombopoiesis. Thus, our results offer insight into the cellular evolution of platelets/MKs in vertebrates. (200/200).
UR - http://www.scopus.com/inward/record.url?scp=84952014384&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84952014384&partnerID=8YFLogxK
U2 - 10.1038/srep18519
DO - 10.1038/srep18519
M3 - Article
C2 - 26687619
AN - SCOPUS:84952014384
SN - 2045-2322
VL - 5
JO - Scientific reports
JF - Scientific reports
M1 - 18519
ER -