Thrombopoietin stimulates proliferation and megakaryocytic differentiation of mouse pro-B cell line BF-TE22

Hideya Ohashi; Haruhiko Morita; Tomoyuki Tahara; Hikaru Tsunakawa; Atsushi Matsumoto; Kinya Ogami; Takashi Kato; Hiroshi Miyazaki

doi:10.1023/A:1007915809529

Thrombopoietin stimulates proliferation and megakaryocytic differentiation of mouse pro-B cell line BF-TE22

Hideya Ohashi^*, Haruhiko Morita, Tomoyuki Tahara, Hikaru Tsunakawa, Atsushi Matsumoto, Kinya Ogami, Takashi Kato, Hiroshi Miyazaki

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

We have isolated and characterized a thrombopoietin (TPO)-dependent BF-TE22 cell line endogenously expressing murine Mp1, which is a subclone of murine pro-B Ba/F3 cells. TPO stimulated the proliferation of BF-TE22 cells in a dose-dependent manner, and also induced the expression of megakaryocyte lineage-specific AP-51 and CD61 cell surface antigens. The results indicate that the murine Mp1 on BF-TE22 cells can transmit both proliferation and megakaryocyte lineage-specific differentiation signals to cells. Furthermore, it was shown that IL-3 inhibits the TPO-induced differentiation signals of BF-TE22 cells. These results suggest that the signals mediated by IL-3 predominate over thor of TPO in BF-TE22 cells. Thus, BF-TE22 cells will be useful for the biological and biochemical studies of the TPO-Mp1 signal transduction mechanism.

Original language	English
Pages (from-to)	199-206
Number of pages	8
Journal	Cytotechnology
Volume	26
Issue number	3
DOIs	https://doi.org/10.1023/A:1007915809529
Publication status	Published - 1998
Externally published	Yes

Keywords

Differentiation
Proliferation
Thrombopoietin

ASJC Scopus subject areas

Biotechnology
Bioengineering
Biomedical Engineering
Clinical Biochemistry
Cell Biology

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@article{74acc95fc38d43a19dfedfb7fd00ff99,

title = "Thrombopoietin stimulates proliferation and megakaryocytic differentiation of mouse pro-B cell line BF-TE22",

abstract = "We have isolated and characterized a thrombopoietin (TPO)-dependent BF-TE22 cell line endogenously expressing murine Mp1, which is a subclone of murine pro-B Ba/F3 cells. TPO stimulated the proliferation of BF-TE22 cells in a dose-dependent manner, and also induced the expression of megakaryocyte lineage-specific AP-51 and CD61 cell surface antigens. The results indicate that the murine Mp1 on BF-TE22 cells can transmit both proliferation and megakaryocyte lineage-specific differentiation signals to cells. Furthermore, it was shown that IL-3 inhibits the TPO-induced differentiation signals of BF-TE22 cells. These results suggest that the signals mediated by IL-3 predominate over thor of TPO in BF-TE22 cells. Thus, BF-TE22 cells will be useful for the biological and biochemical studies of the TPO-Mp1 signal transduction mechanism.",

keywords = "Differentiation, Proliferation, Thrombopoietin",

author = "Hideya Ohashi and Haruhiko Morita and Tomoyuki Tahara and Hikaru Tsunakawa and Atsushi Matsumoto and Kinya Ogami and Takashi Kato and Hiroshi Miyazaki",

year = "1998",

doi = "10.1023/A:1007915809529",

language = "English",

volume = "26",

pages = "199--206",

journal = "Cytotechnology",

issn = "0920-9069",

publisher = "Springer Netherlands",

number = "3",

}

TY - JOUR

T1 - Thrombopoietin stimulates proliferation and megakaryocytic differentiation of mouse pro-B cell line BF-TE22

AU - Ohashi, Hideya

AU - Morita, Haruhiko

AU - Tahara, Tomoyuki

AU - Tsunakawa, Hikaru

AU - Matsumoto, Atsushi

AU - Ogami, Kinya

AU - Kato, Takashi

AU - Miyazaki, Hiroshi

PY - 1998

Y1 - 1998

N2 - We have isolated and characterized a thrombopoietin (TPO)-dependent BF-TE22 cell line endogenously expressing murine Mp1, which is a subclone of murine pro-B Ba/F3 cells. TPO stimulated the proliferation of BF-TE22 cells in a dose-dependent manner, and also induced the expression of megakaryocyte lineage-specific AP-51 and CD61 cell surface antigens. The results indicate that the murine Mp1 on BF-TE22 cells can transmit both proliferation and megakaryocyte lineage-specific differentiation signals to cells. Furthermore, it was shown that IL-3 inhibits the TPO-induced differentiation signals of BF-TE22 cells. These results suggest that the signals mediated by IL-3 predominate over thor of TPO in BF-TE22 cells. Thus, BF-TE22 cells will be useful for the biological and biochemical studies of the TPO-Mp1 signal transduction mechanism.

AB - We have isolated and characterized a thrombopoietin (TPO)-dependent BF-TE22 cell line endogenously expressing murine Mp1, which is a subclone of murine pro-B Ba/F3 cells. TPO stimulated the proliferation of BF-TE22 cells in a dose-dependent manner, and also induced the expression of megakaryocyte lineage-specific AP-51 and CD61 cell surface antigens. The results indicate that the murine Mp1 on BF-TE22 cells can transmit both proliferation and megakaryocyte lineage-specific differentiation signals to cells. Furthermore, it was shown that IL-3 inhibits the TPO-induced differentiation signals of BF-TE22 cells. These results suggest that the signals mediated by IL-3 predominate over thor of TPO in BF-TE22 cells. Thus, BF-TE22 cells will be useful for the biological and biochemical studies of the TPO-Mp1 signal transduction mechanism.

KW - Differentiation

KW - Proliferation

KW - Thrombopoietin

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U2 - 10.1023/A:1007915809529

DO - 10.1023/A:1007915809529

M3 - Article

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AN - SCOPUS:0031900870

SN - 0920-9069

VL - 26

SP - 199

EP - 206

JO - Cytotechnology

JF - Cytotechnology

IS - 3

ER -

Thrombopoietin stimulates proliferation and megakaryocytic differentiation of mouse pro-B cell line BF-TE22

Abstract

Keywords

ASJC Scopus subject areas

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