@article{afe1245fd9984a4dbde549b4795ec673,
title = "Tweezing the cofactor preference of gymnosperm pinene synthase",
abstract = "The cellular activities of gymnosperms monoterpene synthases are largely compromised due to their requirement for manganese, which is deficient in microbial cells. Through site-saturation mutagenesis of the residue adjacent to metal-binding glutamate, we found that pinene synthase is highly mutable at this position yet drastically alter their metal binding preference, thereby quickly improving the cellular performance in heterologous hosts.",
keywords = "Metabolic engineering, Metal dependency, Protein engineering, Synthetic biology",
author = "Miki Tashiro and Koyo Ono and Yuki Kimura and Shigeko Kawai-Noma and Kyoichi Saito and Daisuke Umeno",
note = "Funding Information: D.U. is supported by the PRESTO (JST), Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology [JSPS KAKENHI Grant 15H04189, 15K14228, and 16K21725]. This work is also sup-portedbyTheHamaguchiFoundationfortheAdvancementof Biochemistry, the Futaba Electronics Memorial Foundation, and the Shorai Foundation for Science and Technology. Y.K. is supported by a JSPS fellowship for young scientists. Funding Information: D.U. is supported by the PRESTO (JST), Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology [JSPS KAKENHI Grant 15H04189, 15K14228, and 16K21725]. This work is also supported by The Hamaguchi Foundation for the Advancement of Biochemistry, the Futaba Electronics Memorial Foundation, and the Shorai Foundation for Science and Technology. Y.K. is supported by a JSPS fellowship for young scientists. Publisher Copyright: {\textcopyright} 2018 Japan society for Bioscience, Biotechnology, and agrochemistry",
year = "2018",
doi = "10.1080/09168451.2018.1459465",
language = "English",
volume = "82",
pages = "1058--1061",
journal = "Bioscience, Biotechnology and Biochemistry",
issn = "0916-8451",
publisher = "Japan Society for Bioscience Biotechnology and Agrochemistry",
number = "6",
}