Ultra-sensitive liquid biopsy of circulating extracellular vesicles using ExoScreen

Yusuke Yoshioka; Nobuyoshi Kosaka; Yuki Konishi; Hideki Ohta; Hiroyuki Okamoto; Hikaru Sonoda; Ryoji Nonaka; Hirofumi Yamamoto; Hideshi Ishii; Masaki Mori; Koh Furuta; Takeshi Nakajima; Hiroshi Hayashi; Hajime Sugisaki; Hiroko Higashimoto; Takashi Kato; Fumitaka Takeshita; Takahiro Ochiya

doi:10.1038/ncomms4591

Ultra-sensitive liquid biopsy of circulating extracellular vesicles using ExoScreen

Yusuke Yoshioka, Nobuyoshi Kosaka, Yuki Konishi, Hideki Ohta, Hiroyuki Okamoto, Hikaru Sonoda, Ryoji Nonaka, Hirofumi Yamamoto, Hideshi Ishii, Masaki Mori, Koh Furuta, Takeshi Nakajima, Hiroshi Hayashi, Hajime Sugisaki, Hiroko Higashimoto, Takashi Kato, Fumitaka Takeshita, Takahiro Ochiya

Research output: Contribution to journal › Article › peer-review

382 Citations (Scopus)

Abstract

Cancer cells secrete small membranous extracellular vesicles (EVs) into their microenvironment and circulation. Although their potential as cancer biomarkers has been promising, the identification and quantification of EVs in clinical samples remains challenging. Here we describe a sensitive and rapid analytical technique for profiling circulating EVs directly from blood samples of patients with colorectal cancer. EVs are captured by two types of antibodies and are detected by photosensitizer-beads, which enables us to detect cancer-derived EVs without a purification step. We also show that circulating EVs can be used for detection of colorectal cancer using the antigen CD147, which is embedded in cancer-linked EVs. This work describes a new liquid biopsy technique to sensitively detect disease-specific circulating EVs and provides perspectives in translational medicine from the standpoint of diagnosis and therapy.

Original language	English
Article number	3591
Pages (from-to)	3591
Number of pages	1
Journal	Nature communications
Volume	5
DOIs	https://doi.org/10.1038/ncomms4591
Publication status	Published - 2014
Externally published	Yes

ASJC Scopus subject areas

Chemistry(all)
Biochemistry, Genetics and Molecular Biology(all)
Physics and Astronomy(all)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1038/ncomms4591

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Yoshioka, Y., Kosaka, N., Konishi, Y., Ohta, H., Okamoto, H., Sonoda, H., Nonaka, R., Yamamoto, H., Ishii, H., Mori, M., Furuta, K., Nakajima, T., Hayashi, H., Sugisaki, H., Higashimoto, H., Kato, T., Takeshita, F., & Ochiya, T. (2014). Ultra-sensitive liquid biopsy of circulating extracellular vesicles using ExoScreen. Nature communications, 5, 3591. [3591]. https://doi.org/10.1038/ncomms4591

Yoshioka, Y, Kosaka, N, Konishi, Y, Ohta, H, Okamoto, H, Sonoda, H, Nonaka, R, Yamamoto, H, Ishii, H, Mori, M, Furuta, K, Nakajima, T, Hayashi, H, Sugisaki, H, Higashimoto, H, Kato, T, Takeshita, F & Ochiya, T 2014, 'Ultra-sensitive liquid biopsy of circulating extracellular vesicles using ExoScreen', Nature communications, vol. 5, 3591, pp. 3591. https://doi.org/10.1038/ncomms4591

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title = "Ultra-sensitive liquid biopsy of circulating extracellular vesicles using ExoScreen",

abstract = "Cancer cells secrete small membranous extracellular vesicles (EVs) into their microenvironment and circulation. Although their potential as cancer biomarkers has been promising, the identification and quantification of EVs in clinical samples remains challenging. Here we describe a sensitive and rapid analytical technique for profiling circulating EVs directly from blood samples of patients with colorectal cancer. EVs are captured by two types of antibodies and are detected by photosensitizer-beads, which enables us to detect cancer-derived EVs without a purification step. We also show that circulating EVs can be used for detection of colorectal cancer using the antigen CD147, which is embedded in cancer-linked EVs. This work describes a new liquid biopsy technique to sensitively detect disease-specific circulating EVs and provides perspectives in translational medicine from the standpoint of diagnosis and therapy. ",

author = "Yusuke Yoshioka and Nobuyoshi Kosaka and Yuki Konishi and Hideki Ohta and Hiroyuki Okamoto and Hikaru Sonoda and Ryoji Nonaka and Hirofumi Yamamoto and Hideshi Ishii and Masaki Mori and Koh Furuta and Takeshi Nakajima and Hiroshi Hayashi and Hajime Sugisaki and Hiroko Higashimoto and Takashi Kato and Fumitaka Takeshita and Takahiro Ochiya",

note = "Funding Information: This work was supported in part by a Grant-in-Aid for the Third-Term Comprehensive 10-Year Strategy for Cancer Control, a Grant-in-Aid for Scientific Research on Priority Areas Cancer from the Ministry of Education, Culture, Sports, Science and Technology, and the Program for Promotion of Fundamental Studies in Health Sciences of the National Institute of Biomedical Innovation (NiBio), and the Japan Society for the Promotion of Science (JSPS) through the {\textquoteleft}Funding Program for World-Leading Innovative R&D on Science and Technology (FIRST Program){\textquoteright} initiated by the Council for Science and Technology Policy (CSTP), and a Grant-in-aid for Project for Development of Innovative Research on Cancer Therapeutics (P-Direct), and Grant-in-Aid for Scientific Research on Innovative Areas ({\textquoteleft}functional machinery for non-coding RNAs{\textquoteright}) from the Japanese Ministry of Education, Culture, Sports, Science, and Technology, and Comprehensive Research and Development of a Surgical Instrument for Early Detection and Rapid Curing of Cancer Project (P10003) of the New Energy and IndustrialTechnology Development Organization (NEDO), a research program of the Project for Development of Innovative Research on Cancer Therapeutics (P-Direct), Ministry of Education, Culture, Sports, Science and Technology of Japan. We thank Ms. Ayako Irie at Quantum design Japan for supporting the Nanosight tracking analysis. We thank Ms. Ayako Inoue for excellent technical assistance. We thank Dr Roger Bosse and Dr Nami Kamura at Perkin Elmer for advising protocol for AlphaLisa system.",

year = "2014",

doi = "10.1038/ncomms4591",

language = "English",

volume = "5",

pages = "3591",

journal = "Nature Communications",

issn = "2041-1723",

publisher = "Nature Publishing Group",

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T1 - Ultra-sensitive liquid biopsy of circulating extracellular vesicles using ExoScreen

AU - Yoshioka, Yusuke

AU - Kosaka, Nobuyoshi

AU - Konishi, Yuki

AU - Ohta, Hideki

AU - Okamoto, Hiroyuki

AU - Sonoda, Hikaru

AU - Nonaka, Ryoji

AU - Yamamoto, Hirofumi

AU - Ishii, Hideshi

AU - Mori, Masaki

AU - Furuta, Koh

AU - Nakajima, Takeshi

AU - Hayashi, Hiroshi

AU - Sugisaki, Hajime

AU - Higashimoto, Hiroko

AU - Kato, Takashi

AU - Takeshita, Fumitaka

AU - Ochiya, Takahiro

N1 - Funding Information: This work was supported in part by a Grant-in-Aid for the Third-Term Comprehensive 10-Year Strategy for Cancer Control, a Grant-in-Aid for Scientific Research on Priority Areas Cancer from the Ministry of Education, Culture, Sports, Science and Technology, and the Program for Promotion of Fundamental Studies in Health Sciences of the National Institute of Biomedical Innovation (NiBio), and the Japan Society for the Promotion of Science (JSPS) through the ‘Funding Program for World-Leading Innovative R&D on Science and Technology (FIRST Program)’ initiated by the Council for Science and Technology Policy (CSTP), and a Grant-in-aid for Project for Development of Innovative Research on Cancer Therapeutics (P-Direct), and Grant-in-Aid for Scientific Research on Innovative Areas (‘functional machinery for non-coding RNAs’) from the Japanese Ministry of Education, Culture, Sports, Science, and Technology, and Comprehensive Research and Development of a Surgical Instrument for Early Detection and Rapid Curing of Cancer Project (P10003) of the New Energy and IndustrialTechnology Development Organization (NEDO), a research program of the Project for Development of Innovative Research on Cancer Therapeutics (P-Direct), Ministry of Education, Culture, Sports, Science and Technology of Japan. We thank Ms. Ayako Irie at Quantum design Japan for supporting the Nanosight tracking analysis. We thank Ms. Ayako Inoue for excellent technical assistance. We thank Dr Roger Bosse and Dr Nami Kamura at Perkin Elmer for advising protocol for AlphaLisa system.

PY - 2014

Y1 - 2014

N2 - Cancer cells secrete small membranous extracellular vesicles (EVs) into their microenvironment and circulation. Although their potential as cancer biomarkers has been promising, the identification and quantification of EVs in clinical samples remains challenging. Here we describe a sensitive and rapid analytical technique for profiling circulating EVs directly from blood samples of patients with colorectal cancer. EVs are captured by two types of antibodies and are detected by photosensitizer-beads, which enables us to detect cancer-derived EVs without a purification step. We also show that circulating EVs can be used for detection of colorectal cancer using the antigen CD147, which is embedded in cancer-linked EVs. This work describes a new liquid biopsy technique to sensitively detect disease-specific circulating EVs and provides perspectives in translational medicine from the standpoint of diagnosis and therapy.

AB - Cancer cells secrete small membranous extracellular vesicles (EVs) into their microenvironment and circulation. Although their potential as cancer biomarkers has been promising, the identification and quantification of EVs in clinical samples remains challenging. Here we describe a sensitive and rapid analytical technique for profiling circulating EVs directly from blood samples of patients with colorectal cancer. EVs are captured by two types of antibodies and are detected by photosensitizer-beads, which enables us to detect cancer-derived EVs without a purification step. We also show that circulating EVs can be used for detection of colorectal cancer using the antigen CD147, which is embedded in cancer-linked EVs. This work describes a new liquid biopsy technique to sensitively detect disease-specific circulating EVs and provides perspectives in translational medicine from the standpoint of diagnosis and therapy.

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DO - 10.1038/ncomms4591

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SP - 3591

JO - Nature Communications

JF - Nature Communications

M1 - 3591

ER -

Ultra-sensitive liquid biopsy of circulating extracellular vesicles using ExoScreen

Abstract

ASJC Scopus subject areas

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