Urea biosensor based on the composite film of electroinactive polypyrrole and urease modified with polyanion

Shinichi Komaba; Michiko Seyama; Katsuhisa Tanabe; Tetsuya Osaka

doi:10.5796/kogyobutsurikagaku.64.1228

Urea biosensor based on the composite film of electroinactive polypyrrole and urease modified with polyanion

Shinichi Komaba, Michiko Seyama, Katsuhisa Tanabe, Tetsuya Osaka^*

^*Corresponding author for this work

Research Organization for Nano & Life Innovation

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

A urea sensor based on the composite film of electroinactive polypyrrole (PPy) and urease (Urs) modified with polyanion was fabricated by electropolymerization. This sensor showed the potential response to urea based on the detection of pH-change during the enzymatic reaction by Urs. The sensitivity of this sensor was 53 mV decade^-1 which was higher than that of the PPy film coated electrode in which Urs was entrapped physically as previously reported. The high sensitivity was considered to be attributed to the increase in the number of immobilized Urs with the covalent bonding with carboxyl groups of PAA. This urea sensor showed linear potential response between 1 × 10^-4 mol dm^-3 and 1 × 10^-1 mol dm^-3.

Original language	English
Pages (from-to)	1228-1233
Number of pages	6
Journal	Denki Kagaku
Volume	64
Issue number	12
DOIs	https://doi.org/10.5796/kogyobutsurikagaku.64.1228
Publication status	Published - 1996

Keywords

Biosensor
Electropolymorization
Polypyrrole
Urea sensor

ASJC Scopus subject areas

Chemistry(all)

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10.5796/kogyobutsurikagaku.64.1228

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title = "Urea biosensor based on the composite film of electroinactive polypyrrole and urease modified with polyanion",

abstract = "A urea sensor based on the composite film of electroinactive polypyrrole (PPy) and urease (Urs) modified with polyanion was fabricated by electropolymerization. This sensor showed the potential response to urea based on the detection of pH-change during the enzymatic reaction by Urs. The sensitivity of this sensor was 53 mV decade-1 which was higher than that of the PPy film coated electrode in which Urs was entrapped physically as previously reported. The high sensitivity was considered to be attributed to the increase in the number of immobilized Urs with the covalent bonding with carboxyl groups of PAA. This urea sensor showed linear potential response between 1 × 10-4 mol dm-3 and 1 × 10-1 mol dm-3.",

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T1 - Urea biosensor based on the composite film of electroinactive polypyrrole and urease modified with polyanion

AU - Komaba, Shinichi

AU - Seyama, Michiko

AU - Tanabe, Katsuhisa

AU - Osaka, Tetsuya

PY - 1996

Y1 - 1996

N2 - A urea sensor based on the composite film of electroinactive polypyrrole (PPy) and urease (Urs) modified with polyanion was fabricated by electropolymerization. This sensor showed the potential response to urea based on the detection of pH-change during the enzymatic reaction by Urs. The sensitivity of this sensor was 53 mV decade-1 which was higher than that of the PPy film coated electrode in which Urs was entrapped physically as previously reported. The high sensitivity was considered to be attributed to the increase in the number of immobilized Urs with the covalent bonding with carboxyl groups of PAA. This urea sensor showed linear potential response between 1 × 10-4 mol dm-3 and 1 × 10-1 mol dm-3.

AB - A urea sensor based on the composite film of electroinactive polypyrrole (PPy) and urease (Urs) modified with polyanion was fabricated by electropolymerization. This sensor showed the potential response to urea based on the detection of pH-change during the enzymatic reaction by Urs. The sensitivity of this sensor was 53 mV decade-1 which was higher than that of the PPy film coated electrode in which Urs was entrapped physically as previously reported. The high sensitivity was considered to be attributed to the increase in the number of immobilized Urs with the covalent bonding with carboxyl groups of PAA. This urea sensor showed linear potential response between 1 × 10-4 mol dm-3 and 1 × 10-1 mol dm-3.

KW - Biosensor

KW - Electropolymorization

KW - Polypyrrole

KW - Urea sensor

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Urea biosensor based on the composite film of electroinactive polypyrrole and urease modified with polyanion

Abstract

Keywords

ASJC Scopus subject areas

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