Using Micromanipulation to Analyze Control of Vertebrate Meiotic Spindle Size

Jun Takagi, Takeshi Itabashi, Kazuya Suzuki, Tarun M. Kapoor, Yuta Shimamoto, Shin'ichi Ishiwata*

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    9 Citations (Scopus)


    The polymerization/depolymerization dynamics of microtubules (MTs) have been reported to contribute to control of the size and shape of spindles, but quantitative analysis of how the size and shape correlate with the amount and density of MTs in the spindle remains incomplete. Here, we measured these parameters using 3D microscopy of meiotic spindles thatself-organized in Xenopus egg extracts and presented a simple equation describing the relationship among these parameters. To examine the validity of the equation, we cut the spindle into two fragments along the pole-to-pole axis by micromanipulation techniques that rapidly decrease the amount of MTs. The spheroidal shape spontaneously recovered within 5min, but the size of each fragment remained small. The equation we obtained quantitatively describes how the spindle size correlates with the amount of MTs while maintaining the shape and the MT density

    Original languageEnglish
    Pages (from-to)44-50
    Number of pages7
    JournalCell Reports
    Issue number1
    Publication statusPublished - 2013 Oct 17

    ASJC Scopus subject areas

    • General Biochemistry,Genetics and Molecular Biology


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