Variable domain antibodies specific for viral hemorrhagic septicemia virus (VHSV) selected from a randomized IgNAR phage display library

Maki Ohtani; Jun ichi Hikima; Tae Sung Jung; Hidehiro Kondo; Ikuo Hirono; Haruko Takeyama; Takashi Aoki

doi:10.1016/j.fsi.2012.11.041

Variable domain antibodies specific for viral hemorrhagic septicemia virus (VHSV) selected from a randomized IgNAR phage display library

Maki Ohtani, Jun ichi Hikima, Tae Sung Jung, Hidehiro Kondo^*, Ikuo Hirono, Haruko Takeyama, Takashi Aoki

^*Corresponding author for this work

School of Advanced Science and Engineering

Research output: Contribution to journal › Article › peer-review

17 Citations (Scopus)

Abstract

Phage display libraries are used to screen for nucleotide sequences that encode immunoglobulin variable (V) regions that are specific for a target antigen. We previously constructed an immunoglobulin new antigen receptor (IgNAR) phage display library. Here we used this library to obtain an IgNAR V region that is specific for viral hemorrhagic septicemia virus (VHSV). A phage clone (clone 653) was found to be specific for VHSV by the biopanning method. The V region of clone 653 was used to construct a 6 × His tagged recombinant IgNAR-653 V protein (rIgNAR-653) using the Escherichia coli pET system. The rIgNAR-653 protein bound specifically to VHSV, confirming its activity.

Original language	English
Pages (from-to)	724-728
Number of pages	5
Journal	Fish and Shellfish Immunology
Volume	34
Issue number	2
DOIs	https://doi.org/10.1016/j.fsi.2012.11.041
Publication status	Published - 2013 Feb

Keywords

IgNAR
Neutralization activity
Phage display library
VHSV

ASJC Scopus subject areas

Environmental Chemistry
Aquatic Science

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10.1016/j.fsi.2012.11.041

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title = "Variable domain antibodies specific for viral hemorrhagic septicemia virus (VHSV) selected from a randomized IgNAR phage display library",

abstract = "Phage display libraries are used to screen for nucleotide sequences that encode immunoglobulin variable (V) regions that are specific for a target antigen. We previously constructed an immunoglobulin new antigen receptor (IgNAR) phage display library. Here we used this library to obtain an IgNAR V region that is specific for viral hemorrhagic septicemia virus (VHSV). A phage clone (clone 653) was found to be specific for VHSV by the biopanning method. The V region of clone 653 was used to construct a 6 × His tagged recombinant IgNAR-653 V protein (rIgNAR-653) using the Escherichia coli pET system. The rIgNAR-653 protein bound specifically to VHSV, confirming its activity.",

keywords = "IgNAR, Neutralization activity, Phage display library, VHSV",

author = "Maki Ohtani and Hikima, {Jun ichi} and Jung, {Tae Sung} and Hidehiro Kondo and Ikuo Hirono and Haruko Takeyama and Takashi Aoki",

note = "Funding Information: This work was supported by Grant-in-Aid for Scientific research [KAKENHI KIBAN KENKYU (A) 21248025 ] from the Ministry of Education, Culture, Sports, Science and Technology, Japan . This research was also supported by a grant from the World Class University Program ( R32-10253 ), funded by the Korean Ministry of Education, Science, and Technology of South Korea .",

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AU - Ohtani, Maki

AU - Hikima, Jun ichi

AU - Jung, Tae Sung

AU - Kondo, Hidehiro

AU - Hirono, Ikuo

AU - Takeyama, Haruko

AU - Aoki, Takashi

N1 - Funding Information: This work was supported by Grant-in-Aid for Scientific research [KAKENHI KIBAN KENKYU (A) 21248025 ] from the Ministry of Education, Culture, Sports, Science and Technology, Japan . This research was also supported by a grant from the World Class University Program ( R32-10253 ), funded by the Korean Ministry of Education, Science, and Technology of South Korea .

PY - 2013/2

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AB - Phage display libraries are used to screen for nucleotide sequences that encode immunoglobulin variable (V) regions that are specific for a target antigen. We previously constructed an immunoglobulin new antigen receptor (IgNAR) phage display library. Here we used this library to obtain an IgNAR V region that is specific for viral hemorrhagic septicemia virus (VHSV). A phage clone (clone 653) was found to be specific for VHSV by the biopanning method. The V region of clone 653 was used to construct a 6 × His tagged recombinant IgNAR-653 V protein (rIgNAR-653) using the Escherichia coli pET system. The rIgNAR-653 protein bound specifically to VHSV, confirming its activity.

KW - IgNAR

KW - Neutralization activity

KW - Phage display library

KW - VHSV

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Variable domain antibodies specific for viral hemorrhagic septicemia virus (VHSV) selected from a randomized IgNAR phage display library

Abstract

Keywords

ASJC Scopus subject areas

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