Virus inactivation in hemoglobin solution by heat treatment

H. Abe, K. Ikebuchi, J. Hirayama, M. Fujihara, S. Takeoka*, H. Sakai, E. Tsuchida, H. Ikeda

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)


To increase the safety of stroma-free hemoglobin solution (SFH) as an artificial oxygen carrier source, we investigated the effect of heat treatment on virus inactivation in hemoglobin solution. The hemoglobin solution spiked with vesicular stomatitis virus (VSV) was treated at 60°C for 1 hr under either an air or CO atmosphere. VSV was inactivated at >5.8 log10 and >6.0 log 10under the air and CO atmosphere, respectively. Although the methemoglobin rate increased after the heat treatment under the air atmosphere, no methemoglobin formation was observed by the treatment under the CO atmosphere. Isoelectric focusing analysis revealed the denaturation of hemoglobin after the heat treatment under the air, while hemoglobin banding was not altered in the carbonylated condition. Some protein bands other than hemoglobin were weakened or disappeared on SDS-PAGE after the heat treatment under both con ditions. In addition, the hemoglobin concentration in the SFH was higher after the heat treatment than before the treatment. These findings indicate that the heat treatment under the CO atmosphere inactivates viruses without hemoglobin denaturation, and hence, this method is a promising approach to prepare a safer SFH as artificial oxygen carriers.

Original languageEnglish
Pages (from-to)381-388
Number of pages8
JournalArtificial Cells, Blood Substitutes, and Immobilization Biotechnology
Issue number5
Publication statusPublished - 2001

ASJC Scopus subject areas

  • Biotechnology
  • Biomedical Engineering


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