TY - JOUR
T1 - Virus inactivation in hemoglobin solution by heat treatment
AU - Abe, H.
AU - Ikebuchi, K.
AU - Hirayama, J.
AU - Fujihara, M.
AU - Takeoka, S.
AU - Sakai, H.
AU - Tsuchida, E.
AU - Ikeda, H.
N1 - Funding Information:
This study was supported, in part, by a Health Science Research Grant (Artificial Blood Project) from the Ministry of Health and Welfare, Japan, and by Grants-in-Aid for Scientific Research from the Japanese Ministry of Education, Science, Sports and Culture.
PY - 2001
Y1 - 2001
N2 - To increase the safety of stroma-free hemoglobin solution (SFH) as an artificial oxygen carrier source, we investigated the effect of heat treatment on virus inactivation in hemoglobin solution. The hemoglobin solution spiked with vesicular stomatitis virus (VSV) was treated at 60°C for 1 hr under either an air or CO atmosphere. VSV was inactivated at >5.8 log10 and >6.0 log 10under the air and CO atmosphere, respectively. Although the methemoglobin rate increased after the heat treatment under the air atmosphere, no methemoglobin formation was observed by the treatment under the CO atmosphere. Isoelectric focusing analysis revealed the denaturation of hemoglobin after the heat treatment under the air, while hemoglobin banding was not altered in the carbonylated condition. Some protein bands other than hemoglobin were weakened or disappeared on SDS-PAGE after the heat treatment under both con ditions. In addition, the hemoglobin concentration in the SFH was higher after the heat treatment than before the treatment. These findings indicate that the heat treatment under the CO atmosphere inactivates viruses without hemoglobin denaturation, and hence, this method is a promising approach to prepare a safer SFH as artificial oxygen carriers.
AB - To increase the safety of stroma-free hemoglobin solution (SFH) as an artificial oxygen carrier source, we investigated the effect of heat treatment on virus inactivation in hemoglobin solution. The hemoglobin solution spiked with vesicular stomatitis virus (VSV) was treated at 60°C for 1 hr under either an air or CO atmosphere. VSV was inactivated at >5.8 log10 and >6.0 log 10under the air and CO atmosphere, respectively. Although the methemoglobin rate increased after the heat treatment under the air atmosphere, no methemoglobin formation was observed by the treatment under the CO atmosphere. Isoelectric focusing analysis revealed the denaturation of hemoglobin after the heat treatment under the air, while hemoglobin banding was not altered in the carbonylated condition. Some protein bands other than hemoglobin were weakened or disappeared on SDS-PAGE after the heat treatment under both con ditions. In addition, the hemoglobin concentration in the SFH was higher after the heat treatment than before the treatment. These findings indicate that the heat treatment under the CO atmosphere inactivates viruses without hemoglobin denaturation, and hence, this method is a promising approach to prepare a safer SFH as artificial oxygen carriers.
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U2 - 10.1081/BIO-100106921
DO - 10.1081/BIO-100106921
M3 - Article
C2 - 11708661
AN - SCOPUS:0034763945
SN - 1073-1199
VL - 29
SP - 381
EP - 388
JO - Artificial Cells, Blood Substitutes, and Immobilization Biotechnology
JF - Artificial Cells, Blood Substitutes, and Immobilization Biotechnology
IS - 5
ER -