Virus removal from hemoglobin solution using planova membrane

Y. Naito; I. Fukutomi; Y. Masada; H. Sakai; S. Takeoka; E. Tsuchida; H. Abe; J. Hirayama; K. Ikebuchi; H. Ikeda

doi:10.1007/s100470200025

Virus removal from hemoglobin solution using planova membrane

Y. Naito, I. Fukutomi, Y. Masada, H. Sakai, S. Takeoka, E. Tsuchida^*, H. Abe, J. Hirayama, K. Ikebuchi, H. Ikeda

^*Corresponding author for this work

School of Advanced Science and Engineering

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

Hemoglobin (Hb) vesicles are artificial oxygen carriers that encapsulate concentrated purified Hb with a phospholipid bilayer membrane. They have been confirmed to have sufficient oxygen-transporting ability. Even though strictly inspected outdated red cells are used as a source of Hb, it is necessary to introduce an additional process that inactivates or removes viruses in the process of Hb purification in order to guarantee the utmost safety from infection. In this study, Hb filtration to remove viruses was tested with Planova 35N and 15N (virus removal fitters with a Bemberg microporous membrane). The permeation flux (LMH) and the permeated ratio of Hb solution ([Hb] = 5.6g/dl) through Planova 35N at 13°C were 361/m²/h and almost 100%, respectively. The values for Planova 15N at 13°C were 151/m²/h and 95%, respectively. The permeation flux increased to 181/m²/h when the temperature was raised to 25°C. Under the same conditions, a high efficiency of removal of a bacteriophage, φX174, was confirmed (>7.7log). These results indicate that Planova 15N is effective for the process of virus removal from Hb solution.

Original language	English
Pages (from-to)	141-145
Number of pages	5
Journal	Journal of Artificial Organs
Volume	5
Issue number	2
DOIs	https://doi.org/10.1007/s100470200025
Publication status	Published - 2002 Jul 17

Keywords

Bemberg microporous membrane
Hb purification
Hemoglobin vesicles
Oxygen carriers
Virus removal

ASJC Scopus subject areas

Medicine (miscellaneous)
Biomaterials
Biomedical Engineering
Cardiology and Cardiovascular Medicine

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10.1007/s100470200025

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title = "Virus removal from hemoglobin solution using planova membrane",

abstract = "Hemoglobin (Hb) vesicles are artificial oxygen carriers that encapsulate concentrated purified Hb with a phospholipid bilayer membrane. They have been confirmed to have sufficient oxygen-transporting ability. Even though strictly inspected outdated red cells are used as a source of Hb, it is necessary to introduce an additional process that inactivates or removes viruses in the process of Hb purification in order to guarantee the utmost safety from infection. In this study, Hb filtration to remove viruses was tested with Planova 35N and 15N (virus removal fitters with a Bemberg microporous membrane). The permeation flux (LMH) and the permeated ratio of Hb solution ([Hb] = 5.6g/dl) through Planova 35N at 13°C were 361/m2/h and almost 100%, respectively. The values for Planova 15N at 13°C were 151/m2/h and 95%, respectively. The permeation flux increased to 181/m2/h when the temperature was raised to 25°C. Under the same conditions, a high efficiency of removal of a bacteriophage, φX174, was confirmed (>7.7log). These results indicate that Planova 15N is effective for the process of virus removal from Hb solution.",

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T1 - Virus removal from hemoglobin solution using planova membrane

AU - Naito, Y.

AU - Fukutomi, I.

AU - Masada, Y.

AU - Sakai, H.

AU - Takeoka, S.

AU - Tsuchida, E.

AU - Abe, H.

AU - Hirayama, J.

AU - Ikebuchi, K.

AU - Ikeda, H.

PY - 2002/7/17

Y1 - 2002/7/17

N2 - Hemoglobin (Hb) vesicles are artificial oxygen carriers that encapsulate concentrated purified Hb with a phospholipid bilayer membrane. They have been confirmed to have sufficient oxygen-transporting ability. Even though strictly inspected outdated red cells are used as a source of Hb, it is necessary to introduce an additional process that inactivates or removes viruses in the process of Hb purification in order to guarantee the utmost safety from infection. In this study, Hb filtration to remove viruses was tested with Planova 35N and 15N (virus removal fitters with a Bemberg microporous membrane). The permeation flux (LMH) and the permeated ratio of Hb solution ([Hb] = 5.6g/dl) through Planova 35N at 13°C were 361/m2/h and almost 100%, respectively. The values for Planova 15N at 13°C were 151/m2/h and 95%, respectively. The permeation flux increased to 181/m2/h when the temperature was raised to 25°C. Under the same conditions, a high efficiency of removal of a bacteriophage, φX174, was confirmed (>7.7log). These results indicate that Planova 15N is effective for the process of virus removal from Hb solution.

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Virus removal from hemoglobin solution using planova membrane

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Keywords

ASJC Scopus subject areas

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