Water-soluble conjugate of double-stranded DNA and poly(N-isopropylacrylamide) for one-pot affinity precipitation separation of DNA-binding proteins

Daisuke Umeno; Masafumi Kawasaki; Mizuo Maeda

doi:10.1021/bc980019f

Water-soluble conjugate of double-stranded DNA and poly(N-isopropylacrylamide) for one-pot affinity precipitation separation of DNA-binding proteins

Daisuke Umeno, Masafumi Kawasaki, Mizuo Maeda^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

50 Citations (Scopus)

Abstract

Poly(N-isopropylacrylamide) having a terminal psoralen group was synthesized and covalently bound to plasmid pBR 322 DNA through a photochemical reaction. The resulting conjugate exhibited temperature-responsive precipitation due to the aggregation of poly(N-isopropylacrylamide) chains when heated above 31 °C. This system was used for the one-pot separation of restriction endonuclease EcoRI.

Original language	English
Pages (from-to)	719-724
Number of pages	6
Journal	Bioconjugate Chemistry
Volume	9
Issue number	6
DOIs	https://doi.org/10.1021/bc980019f
Publication status	Published - 1998
Externally published	Yes

ASJC Scopus subject areas

Biotechnology
Bioengineering
Biomedical Engineering
Pharmacology
Pharmaceutical Science
Organic Chemistry

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10.1021/bc980019f

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title = "Water-soluble conjugate of double-stranded DNA and poly(N-isopropylacrylamide) for one-pot affinity precipitation separation of DNA-binding proteins",

abstract = "Poly(N-isopropylacrylamide) having a terminal psoralen group was synthesized and covalently bound to plasmid pBR 322 DNA through a photochemical reaction. The resulting conjugate exhibited temperature-responsive precipitation due to the aggregation of poly(N-isopropylacrylamide) chains when heated above 31 °C. This system was used for the one-pot separation of restriction endonuclease EcoRI.",

author = "Daisuke Umeno and Masafumi Kawasaki and Mizuo Maeda",

year = "1998",

doi = "10.1021/bc980019f",

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volume = "9",

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journal = "Bioconjugate Chemistry",

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T1 - Water-soluble conjugate of double-stranded DNA and poly(N-isopropylacrylamide) for one-pot affinity precipitation separation of DNA-binding proteins

AU - Umeno, Daisuke

AU - Kawasaki, Masafumi

AU - Maeda, Mizuo

PY - 1998

Y1 - 1998

N2 - Poly(N-isopropylacrylamide) having a terminal psoralen group was synthesized and covalently bound to plasmid pBR 322 DNA through a photochemical reaction. The resulting conjugate exhibited temperature-responsive precipitation due to the aggregation of poly(N-isopropylacrylamide) chains when heated above 31 °C. This system was used for the one-pot separation of restriction endonuclease EcoRI.

AB - Poly(N-isopropylacrylamide) having a terminal psoralen group was synthesized and covalently bound to plasmid pBR 322 DNA through a photochemical reaction. The resulting conjugate exhibited temperature-responsive precipitation due to the aggregation of poly(N-isopropylacrylamide) chains when heated above 31 °C. This system was used for the one-pot separation of restriction endonuclease EcoRI.

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DO - 10.1021/bc980019f

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AN - SCOPUS:0032213085

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JF - Bioconjugate Chemistry

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Water-soluble conjugate of double-stranded DNA and poly(N-isopropylacrylamide) for one-pot affinity precipitation separation of DNA-binding proteins

Abstract

ASJC Scopus subject areas

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