Zeptomole Detection of an Enzyme by a Simple Colorimetric Method Kanako

Kanako Iha; Yuta Kyosei; Mayuri Namba; Daiki Makioka; Sou Yamura; Satoshi Watabe; Teruki Yoshimura; Etsuro Ito

doi:10.2116/analsci.21N009

Zeptomole Detection of an Enzyme by a Simple Colorimetric Method Kanako

Kanako Iha, Yuta Kyosei, Mayuri Namba, Daiki Makioka, Sou Yamura, Satoshi Watabe, Teruki Yoshimura, Etsuro Ito^*

^*Corresponding author for this work

School of Education

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

An enzyme immunoassay, in which an enzyme (e.g., alkaline phosphatase, ALP) is conjugated with an antibody, is a precise and simple protein detection method. Precise measurements of enzymes at low concentrations allow for ultrasensitive protein detection. The application of a phosphorylated substrate to ALP, followed by using a dephosphorylated substrate in thionicotinamide-adenine dinucleotide cycling, provides a simple and precise quantification of ALP. We describe a protocol for detecting ALP at the zeptomole level using a simple colorimetric method.

Original language	English
Pages (from-to)	1469-1472
Number of pages	4
Journal	Analytical Sciences
Volume	37
Issue number	10
DOIs	https://doi.org/10.2116/analsci.21N009
Publication status	Published - 2021

Keywords

Colorimetry
thio-NAD cycling
ultrasensitive ELISA
zeptomole

ASJC Scopus subject areas

Analytical Chemistry

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10.2116/analsci.21N009

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title = "Zeptomole Detection of an Enzyme by a Simple Colorimetric Method Kanako",

abstract = "An enzyme immunoassay, in which an enzyme (e.g., alkaline phosphatase, ALP) is conjugated with an antibody, is a precise and simple protein detection method. Precise measurements of enzymes at low concentrations allow for ultrasensitive protein detection. The application of a phosphorylated substrate to ALP, followed by using a dephosphorylated substrate in thionicotinamide-adenine dinucleotide cycling, provides a simple and precise quantification of ALP. We describe a protocol for detecting ALP at the zeptomole level using a simple colorimetric method.",

keywords = "Colorimetry, thio-NAD cycling, ultrasensitive ELISA, zeptomole",

author = "Kanako Iha and Yuta Kyosei and Mayuri Namba and Daiki Makioka and Sou Yamura and Satoshi Watabe and Teruki Yoshimura and Etsuro Ito",

note = "Funding Information: This work was partly supported by the Waseda University grants for Specific Research Projects (grant numbers 2017A-015 and 2019C-123). Publisher Copyright: {\textcopyright} 2021. The Japan Society for Analytical Chemistry",

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doi = "10.2116/analsci.21N009",

language = "English",

volume = "37",

pages = "1469--1472",

journal = "Analytical Sciences",

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T1 - Zeptomole Detection of an Enzyme by a Simple Colorimetric Method Kanako

AU - Iha, Kanako

AU - Kyosei, Yuta

AU - Namba, Mayuri

AU - Makioka, Daiki

AU - Yamura, Sou

AU - Watabe, Satoshi

AU - Yoshimura, Teruki

AU - Ito, Etsuro

N1 - Funding Information: This work was partly supported by the Waseda University grants for Specific Research Projects (grant numbers 2017A-015 and 2019C-123). Publisher Copyright: © 2021. The Japan Society for Analytical Chemistry

PY - 2021

Y1 - 2021

N2 - An enzyme immunoassay, in which an enzyme (e.g., alkaline phosphatase, ALP) is conjugated with an antibody, is a precise and simple protein detection method. Precise measurements of enzymes at low concentrations allow for ultrasensitive protein detection. The application of a phosphorylated substrate to ALP, followed by using a dephosphorylated substrate in thionicotinamide-adenine dinucleotide cycling, provides a simple and precise quantification of ALP. We describe a protocol for detecting ALP at the zeptomole level using a simple colorimetric method.

AB - An enzyme immunoassay, in which an enzyme (e.g., alkaline phosphatase, ALP) is conjugated with an antibody, is a precise and simple protein detection method. Precise measurements of enzymes at low concentrations allow for ultrasensitive protein detection. The application of a phosphorylated substrate to ALP, followed by using a dephosphorylated substrate in thionicotinamide-adenine dinucleotide cycling, provides a simple and precise quantification of ALP. We describe a protocol for detecting ALP at the zeptomole level using a simple colorimetric method.

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KW - thio-NAD cycling

KW - ultrasensitive ELISA

KW - zeptomole

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Zeptomole Detection of an Enzyme by a Simple Colorimetric Method Kanako

Abstract

Keywords

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