Zirconia substrate with periodic surface microstructures enhances osteogenic differentiation of rat adipose-derived stem cells

Mayu Yasunaga; Tomoko Watanabe; Gen Yano; Kazutoshi Murotomi; Miki Hiramatsu; Motohiro Hirose; Masayuki Kakehata; Hidehiko Yashiro; Atsushi Yamazaki; Atsuo Ito

doi:10.1016/j.matlet.2022.133544

Zirconia substrate with periodic surface microstructures enhances osteogenic differentiation of rat adipose-derived stem cells

Mayu Yasunaga^*, Tomoko Watanabe, Gen Yano, Kazutoshi Murotomi, Miki Hiramatsu, Motohiro Hirose, Masayuki Kakehata, Hidehiko Yashiro, Atsushi Yamazaki, Atsuo Ito

^*Corresponding author for this work

School of Creative Science and Engineering

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

Zirconia substrates with periodic surface microstructures formed by irradiation with a femtosecond laser previously enhanced osteogenic differentiation of rat bone marrow mesenchymal stem cells (BMSCs), associated with a reduction in the cell attachment area. Adipose-derived mesenchymal stem cells (ADSCs) are clinically less difficult in harvesting the cells than BMSCs. In this study, we investigated the effects of zirconia substrates with the periodic surface microstructures on osteogenic differentiation of rat ADSCs. Zirconia substrates with the periodic surface microstructures enhanced not adipogenic differentiation but osteogenic differentiation of rat ADSCs, associated with a reduction in the cell attachment area. Therefore, zirconia substrates with surface periodic microstructures formed by irradiation with a femtosecond laser could be useful in bone tissue engineering using ADSCs when BMSCs are unavailable.

Original language	English
Article number	133544
Journal	Materials Letters
Volume	332
DOIs	https://doi.org/10.1016/j.matlet.2022.133544
Publication status	Published - 2023 Feb 1

Keywords

Adipose-derived mesenchymal stem cells
Cell attachment area
Femtosecond laser irradiation
Osteogenic differentiation
Zirconia substrates

ASJC Scopus subject areas

General Materials Science
Condensed Matter Physics
Mechanics of Materials
Mechanical Engineering

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@article{73f80ab154154a7fba991698a8430d8f,

title = "Zirconia substrate with periodic surface microstructures enhances osteogenic differentiation of rat adipose-derived stem cells",

abstract = "Zirconia substrates with periodic surface microstructures formed by irradiation with a femtosecond laser previously enhanced osteogenic differentiation of rat bone marrow mesenchymal stem cells (BMSCs), associated with a reduction in the cell attachment area. Adipose-derived mesenchymal stem cells (ADSCs) are clinically less difficult in harvesting the cells than BMSCs. In this study, we investigated the effects of zirconia substrates with the periodic surface microstructures on osteogenic differentiation of rat ADSCs. Zirconia substrates with the periodic surface microstructures enhanced not adipogenic differentiation but osteogenic differentiation of rat ADSCs, associated with a reduction in the cell attachment area. Therefore, zirconia substrates with surface periodic microstructures formed by irradiation with a femtosecond laser could be useful in bone tissue engineering using ADSCs when BMSCs are unavailable.",

keywords = "Adipose-derived mesenchymal stem cells, Cell attachment area, Femtosecond laser irradiation, Osteogenic differentiation, Zirconia substrates",

author = "Mayu Yasunaga and Tomoko Watanabe and Gen Yano and Kazutoshi Murotomi and Miki Hiramatsu and Motohiro Hirose and Masayuki Kakehata and Hidehiko Yashiro and Atsushi Yamazaki and Atsuo Ito",

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year = "2023",

month = feb,

day = "1",

doi = "10.1016/j.matlet.2022.133544",

language = "English",

volume = "332",

journal = "Materials Letters",

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T1 - Zirconia substrate with periodic surface microstructures enhances osteogenic differentiation of rat adipose-derived stem cells

AU - Yasunaga, Mayu

AU - Watanabe, Tomoko

AU - Yano, Gen

AU - Murotomi, Kazutoshi

AU - Hiramatsu, Miki

AU - Hirose, Motohiro

AU - Kakehata, Masayuki

AU - Yashiro, Hidehiko

AU - Yamazaki, Atsushi

AU - Ito, Atsuo

PY - 2023/2/1

Y1 - 2023/2/1

N2 - Zirconia substrates with periodic surface microstructures formed by irradiation with a femtosecond laser previously enhanced osteogenic differentiation of rat bone marrow mesenchymal stem cells (BMSCs), associated with a reduction in the cell attachment area. Adipose-derived mesenchymal stem cells (ADSCs) are clinically less difficult in harvesting the cells than BMSCs. In this study, we investigated the effects of zirconia substrates with the periodic surface microstructures on osteogenic differentiation of rat ADSCs. Zirconia substrates with the periodic surface microstructures enhanced not adipogenic differentiation but osteogenic differentiation of rat ADSCs, associated with a reduction in the cell attachment area. Therefore, zirconia substrates with surface periodic microstructures formed by irradiation with a femtosecond laser could be useful in bone tissue engineering using ADSCs when BMSCs are unavailable.

AB - Zirconia substrates with periodic surface microstructures formed by irradiation with a femtosecond laser previously enhanced osteogenic differentiation of rat bone marrow mesenchymal stem cells (BMSCs), associated with a reduction in the cell attachment area. Adipose-derived mesenchymal stem cells (ADSCs) are clinically less difficult in harvesting the cells than BMSCs. In this study, we investigated the effects of zirconia substrates with the periodic surface microstructures on osteogenic differentiation of rat ADSCs. Zirconia substrates with the periodic surface microstructures enhanced not adipogenic differentiation but osteogenic differentiation of rat ADSCs, associated with a reduction in the cell attachment area. Therefore, zirconia substrates with surface periodic microstructures formed by irradiation with a femtosecond laser could be useful in bone tissue engineering using ADSCs when BMSCs are unavailable.

KW - Adipose-derived mesenchymal stem cells

KW - Cell attachment area

KW - Femtosecond laser irradiation

KW - Osteogenic differentiation

KW - Zirconia substrates

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U2 - 10.1016/j.matlet.2022.133544

DO - 10.1016/j.matlet.2022.133544

M3 - Article

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SN - 0167-577X

VL - 332

JO - Materials Letters

JF - Materials Letters

M1 - 133544

ER -

Zirconia substrate with periodic surface microstructures enhances osteogenic differentiation of rat adipose-derived stem cells

Abstract

Keywords

ASJC Scopus subject areas

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