TY - JOUR
T1 - Zirconia substrate with periodic surface microstructures enhances osteogenic differentiation of rat adipose-derived stem cells
AU - Yasunaga, Mayu
AU - Watanabe, Tomoko
AU - Yano, Gen
AU - Murotomi, Kazutoshi
AU - Hiramatsu, Miki
AU - Hirose, Motohiro
AU - Kakehata, Masayuki
AU - Yashiro, Hidehiko
AU - Yamazaki, Atsushi
AU - Ito, Atsuo
N1 - Funding Information:
The authors are grateful to Dr. Kumi Morikawa at National Institute of Advanced Industrial Science and Technology (AIST) for technical assistance in isolation of rat ADSCs. This work was supported by the Japan Society for the Promotion of Science KAKENHI [grant number 19K12815 and 19K05320].
Funding Information:
This work was supported by the Japan Society for the Promotion of Science KAKENHI [grant number 19K12815 and 19K05320 ].
Publisher Copyright:
© 2022 The Author(s)
PY - 2023/2/1
Y1 - 2023/2/1
N2 - Zirconia substrates with periodic surface microstructures formed by irradiation with a femtosecond laser previously enhanced osteogenic differentiation of rat bone marrow mesenchymal stem cells (BMSCs), associated with a reduction in the cell attachment area. Adipose-derived mesenchymal stem cells (ADSCs) are clinically less difficult in harvesting the cells than BMSCs. In this study, we investigated the effects of zirconia substrates with the periodic surface microstructures on osteogenic differentiation of rat ADSCs. Zirconia substrates with the periodic surface microstructures enhanced not adipogenic differentiation but osteogenic differentiation of rat ADSCs, associated with a reduction in the cell attachment area. Therefore, zirconia substrates with surface periodic microstructures formed by irradiation with a femtosecond laser could be useful in bone tissue engineering using ADSCs when BMSCs are unavailable.
AB - Zirconia substrates with periodic surface microstructures formed by irradiation with a femtosecond laser previously enhanced osteogenic differentiation of rat bone marrow mesenchymal stem cells (BMSCs), associated with a reduction in the cell attachment area. Adipose-derived mesenchymal stem cells (ADSCs) are clinically less difficult in harvesting the cells than BMSCs. In this study, we investigated the effects of zirconia substrates with the periodic surface microstructures on osteogenic differentiation of rat ADSCs. Zirconia substrates with the periodic surface microstructures enhanced not adipogenic differentiation but osteogenic differentiation of rat ADSCs, associated with a reduction in the cell attachment area. Therefore, zirconia substrates with surface periodic microstructures formed by irradiation with a femtosecond laser could be useful in bone tissue engineering using ADSCs when BMSCs are unavailable.
KW - Adipose-derived mesenchymal stem cells
KW - Cell attachment area
KW - Femtosecond laser irradiation
KW - Osteogenic differentiation
KW - Zirconia substrates
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U2 - 10.1016/j.matlet.2022.133544
DO - 10.1016/j.matlet.2022.133544
M3 - Article
AN - SCOPUS:85142316098
SN - 0167-577X
VL - 332
JO - Materials Letters
JF - Materials Letters
M1 - 133544
ER -