Daisuke Kiga

2022

Development of an expression-tunable multiple protein synthesis system in cell-free reactions using T7-promoter-variant series

Senda, N., Enomoto, T., Kihara, K., Yamashiro, N., Takagi, N., Kiga, D. & Nishida, H., 2022, In: Synthetic Biology. 7, 1, ysac029.

研究成果: Article › 査読

Open Access

1 被引用数 (Scopus)

Multicolor plate reader fluorescence calibration

Beal, J., Telmer, C. A., Vignoni, A., Boada, Y., Baldwin, G. S., Hallett, L., Lee, T., Selvarajah, V., Billerbeck, S., Brown, B., Cai, G. N., Cai, L., Eisenstein, E., Kiga, D., Ross, D., Alperovich, N., Sprent, N., Thompson, J., Young, E. M., Endy, D., & 1 othersHaddock-Angelli, T., 2022, In: Synthetic Biology. 7, 1, ysac010.

研究成果: Article › 査読

Open Access

1 被引用数 (Scopus)

2021

Comparative analysis of three studies measuring fluorescence from engineered bacterial genetic constructs

The iGEM Interlab Study Contributors, 2021 6月, In: PloS one. 16, 6 June, e0252263.

研究成果: Article › 査読

Open Access

9 被引用数 (Scopus)

遺伝暗号による制約を突破する翻訳システムの改変と非標準アミノ酸の合成・重合

Kiga, D., 2021, In: Seikagaku. 93, 3, p. 281-282 2 p.

研究成果: Article › 査読

遺伝暗号改変を活用した人工進化からみる magic20 の意味

Kiga, D., 2021, In: Seikagaku. 93, 3, p. 359-365 7 p.

研究成果: Article › 査読

2020

Erratum: Author Correction: Robust estimation of bacterial cell count from optical density (Communications biology (2020) 3 1 (512))

iGEM Interlab Study Contributors, 2020 10月 27, In: Communications Biology. 3, 1, p. 640 1 p.

研究成果: Comment/debate › 査読

Open Access

2 被引用数 (Scopus)

Robust estimation of bacterial cell count from optical density

NTNU_Trondheim, NTU-Singapore, NU_Kazakhstan, NUDT_CHINA, NUS_Singapore-A, NUS_Singapore-Sci, NWU-China, NYMU-Taipei, NYU_Abu_Dhabi, OUC-China, Oxford, Paris_Bettencourt, Pasteur_Paris, Peking, Pittsburgh, Purdue, Queens_Canada, RDFZ-China, REC-CHENNAI, Rheda_Bielefeld, & 225 othersRHIT, Rice, Ruia-Mumbai, SBS_SH_112144, SCAU-China, SCU-China, SCUT_ChinaB, SCUT-ChinaA, SDU-CHINA, SFLS_Shenzhen, ShanghaiTech, SHSBNU_China, SHSID_China, SHSU_China, SIAT-SCIE, SJTU-BioX-Shanghai, SKLMT-China, SMMU-China, SMS_Shenzhen, Sorbonne_U_Paris, SSHS-Shenzhen, SSTi-SZGD, St_Andrews, Stanford, Stanford-Brown-RISD, Stockholm, Stony_Brook, Stuttgart, SUIS_Shanghai, SYSU-CHINA, SYSU-Software, SZU-China, Tacoma_RAINmakers, Tartu_TUIT, TAS_Taipei, Tec-Chihuahua, Tec-Monterrey, TecCEM, TecMonterrey_GDL, Thessaloniki, Tongji_China, Toronto, Toulouse-INSA-UPS, TU_Darmstadt, TU-Eindhoven, TUDelft, TUST_China, UAlberta, UC_Davis, UC_San_Diego, UCAS-China, UChicago, UChile_Biotec, UCL, UCLouvain, UConn, UCopenhagen, UESTC-China, UFlorida, UGA, UI_Indonesia, UiOslo_Norway, UIOWA, UIUC_Illinois, ULaval, ULaVerne_Collab, UMaryland, UNebraska-Lincoln, Unesp_Brazil, UNSW_Australia, UPF_CRG_Barcelona, UPF_CRG_Barcelona, US_AFRL_CarrollHS, USMA-West_Point, USP-Brazil, UST_Beijing, USTC, UT-Knoxville, Utrecht, Valencia_UPV, Vilnius-Lithuania, Vilnius-Lithuania-OG, Virginia, Warwick, Washington, WashU_StLouis, Waterloo, Westminster_UK, WHU-China, William_and_Mary, Worldshaper-Wuhan, WPI_Worcester, XJTLU-CHINA, XJTU-China, XMU-China, Yale, ZJU-China, ZJUT-China, iGEM Interlab Study Contributors, Aachen, Aalto-Helsinki, AHUT_China, Aix-Marseille, ASTWS-China, Athens, Austin_LASA, Austin_UTexas, Baltimore_BioCrew, BCU, BFSUICC-China, BGIC-Global, BGU_Israel, Bielefeld-CeBiTec, Bilkent-UNAMBG, BioIQS-Barcelona, BioMarvel, BIT, BIT-China, BJRS_China, BNDS_CHINA, BNU-China, BOKU-Vienna, BostonU, British_Columbia, Calgary, Cardiff_Wales, CCU_Taiwan, CDHSU-CHINA, Chalmers-Gothenburg, CIEI-BJ, CMUQ, CO_Mines, ColumbiaNYC, Cornell, CPU_CHINA, CSU_CHINA, CSU_Fort_Collins, Delgado-Ivy-Marin, DLUT_China, DLUT_China_B, DNHS_SanDiego, DTU-Denmark, Duesseldorf, Ecuador, ECUST, Edinburgh_OG, Edinburgh_UG, Emory, EPFL, ETH_Zurich, Evry_Paris-Saclay, Exeter, FAU_Erlangen, FJNU-China, Fudan, Fudan-CHINA, GDSYZX, Georgia_State, Gifu, GO_Paris-Saclay, GreatBay_China, Groningen, GZHS-United, HAFS, Hamburg, HBUT-China, HebrewU, HFLS_ZhejiangUnited, HK_HCY_LFC, HKJS_S, Hong_Kong_HKU, Hong_Kong_HKUST, Hong_Kong_JSS, Hong_Kong-CUHK, HUBU-Wuhan, HUST-China, HZAU-China, ICT-Mumbai, IISc-Bangalore, IISER-Bhopal-India, IISER-Kolkata, IISER-Mohali, IIT_Delhi, IIT_Kanpur, IIT-Madras, Jiangnan_China, Jilin_China, JMU_Wuerzburg, KAIT_JAPAN, KCL_UK, KUAS_Korea, Lambert_GA, Leiden, Lethbridge, Lethbridge_HS, Lubbock_TTU, Lund, Macquarie_Australia, Madrid-OLM, Manchester, Marburg, McGill, McMaster, METU_HS_Ankara, Michigan, MichiganState, Mingdao, Minnesota, Montpellier, Munich, Nanjing-China, NAU-CHINA, NAWI_Graz, NCHU_Taichung, NCTU_Formosa, NEU_China_A, NEU_China_B, Newcastle, NJU-China, NKU_CHINA, Northwestern, Nottingham, NPU-China, NTHU_Formosa & NTHU_Taiwan, 2020 12月, In: Communications Biology. 3, 1, 512.

研究成果: Article › 査読

Open Access

68 被引用数 (Scopus)

The long journey towards standards for engineering biosystems: Are the Molecular Biology and the Biotech communities ready to standardise?

Beal, J., Goñi-Moreno, A., Myers, C., Hecht, A., de Vicente, M. D. C., Parco, M., Schmidt, M., Timmis, K., Baldwin, G., Friedrichs, S., Freemont, P., Kiga, D., Ordozgoiti, E., Rennig, M., Rios, L., Tanner, K., de Lorenzo, V. & Porcar, M., 2020 5月 6, In: EMBO Reports. 21, 5, e50521.

研究成果: Article › 査読

Open Access

35 被引用数 (Scopus)

2019

Comparison between effects of retroactivity and resource competition upon change in downstream reporter genes of synthetic genetic circuits

Moriya, T., Yamaoka, T., Wakayama, Y., Ayukawa, S., Zhang, Z., Yamamura, M., Wakao, S. & Kiga, D., 2019 3月, In: Life. 9, 1, 30.

研究成果: Article › 査読

Open Access

2 被引用数 (Scopus)

Enhancement of binding affinity of folate to its receptor by peptide conjugation

Dharmatti, R., Miyatake, H., Nandakumar, A., Ueda, M., Kobayashi, K., Kiga, D., Yamamura, M. & Ito, Y., 2019 5月 1, In: International journal of molecular sciences. 20, 9, 2152.

研究成果: Article › 査読

Open Access

10 被引用数 (Scopus)

RNA world

Ayukawa, S., Enomoto, T. & Kiga, D., 2019 1月 1, Astrobiology: From the Origins of Life to the Search for Extraterrestrial Intelligence. Springer Singapore, p. 77-90 14 p.

研究成果: Chapter

1 被引用数 (Scopus)

2018

A Highly Bioactive Lys-Deficient IFN Leads to a Site-Specific Di-PEGylated IFN with Equivalent Bioactivity to That of Unmodified IFN-α2b

Imada, T., Moriya, K., Uchiyama, M., Inukai, N., Hitotsuyanagi, M., Masuda, A., Suzuki, T., Ayukawa, S., Tagawa, Y. I., Dohmae, N., Kohara, M., Yamamura, M. & Kiga, D., 2018 11月 16, In: ACS Synthetic Biology. 7, 11, p. 2537-2546 10 p.

研究成果: Article › 査読

Open Access

1 被引用数 (Scopus)

Escherichia coli expression, purification, and refolding of human folate receptor α (hFRα) and β (hFRβ)

Dharmatti, R., Miyatake, H., Zhang, C., Ren, X., Yumoto, A., Kiga, D., Yamamura, M. & Ito, Y., 2018 9月, In: Protein Expression and Purification. 149, p. 17-22 6 p.

研究成果: Article › 査読

3 被引用数 (Scopus)

Horizontal transfer of code fragments between protocells can explain the origins of the genetic code without vertical descent

Froese, T., Campos, J. I., Fujishima, K., Kiga, D. & Virgo, N., 2018 12月 1, In: Scientific reports. 8, 1, 3532.

研究成果: Article › 査読

Open Access

10 被引用数 (Scopus)

2017

Constraint-based perturbation analysis with cluster Newton method: A case study of personalized parameter estimations with irinotecan whole-body physiologically based pharmacokinetic model

Asami, S., Kiga, D. & Konagaya, A., 2017 12月 21, In: BMC Systems Biology. 11, 129.

研究成果: Article › 査読

Open Access

9 被引用数 (Scopus)

2016

A bacterial continuous culture system based on a microfluidic droplet open reactor

Ito, M., Sugiura, H., Ayukawa, S., Kiga, D. & Takinoue, M., 2016, In: Analytical Sciences. 32, 1, p. 61-66 6 p.

研究成果: Article › 査読

4 被引用数 (Scopus)

High-frequency noise attenuation of a two-component system responding to short-pulse input

Nishida, A., Sekine, R., Kiga, D. & Yamamura, M., 2016 12月 19, Proceedings of the 2016 7th International Conference on Computational Systems-Biology and Bioinformatics, CSBio 2016. Association for Computing Machinery, p. 28-35 8 p. (ACM International Conference Proceeding Series).

研究成果: Conference contribution

Open Access

1 被引用数 (Scopus)

Reproducibility of fluorescent expression from engineered biological constructs in E. coli

iGEM Interlab Study Contributors, 2016 3月, In: PloS one. 11, 3, e0150182.

研究成果: Article › 査読

Open Access

32 被引用数 (Scopus)

2015

In vitro selection of a photoresponsive peptide aptamer to glutathione-immobilized microbeads

Tada, S., Zang, Q., Wang, W., Kawamoto, M., Liu, M., Iwashita, M., Uzawa, T., Kiga, D., Yamamura, M. & Ito, Y., 2015 2月 1, In: Journal of Bioscience and Bioengineering. 119, 2, p. 137-139 3 p.

研究成果: Article › 査読

3 被引用数 (Scopus)

Two site genetic incorporation of varying length polyethylene glycol into the backbone of one peptide

Zang, Q., Tada, S., Uzawa, T., Kiga, D., Yamamura, M. & Ito, Y., 2015 7月 31, In: Chemical Communications. 51, 76, p. 14385-14388 4 p.

研究成果: Article › 査読

5 被引用数 (Scopus)

2014

An observation method for autonomous signaling- mediated synthetic diversification in escherichia coli

Sekine, R., Ayukawa, S. & Kiga, D., 2014, In: Methods in Molecular Biology. 1151, p. 69-74 6 p.

研究成果: Article › 査読

Construction and control of synthetic genetic circuits

Sekine, R. & Kiga, D., 2014, In: Seikagaku. 86, 2, p. 201-208 8 p.

研究成果: Review article › 査読

Effects of downstream genes on synthetic genetic circuits

Moriya, T., Yamamura, M. & Kiga, D., 2014 12月 8, In: BMC Systems Biology. 8, 4, S4.

研究成果: Article › 査読

Open Access

6 被引用数 (Scopus)

Experimental Evolution of a Green Fluorescent Protein Composed of 19 Unique Amino Acids without Tryptophan

Kawahara-Kobayashi, A., Hitotsuyanagi, M., Amikura, K. & Kiga, D., 2014 12月 11, In: Origins of Life and Evolution of Biospheres. 44, 2, p. 75-86 12 p.

研究成果: Article › 査読

1 被引用数 (Scopus)

General Applicability of Synthetic Gene-Overexpression for Cell-Type Ratio Control via Reprogramming

Ishimatsu, K., Hata, T., Mochizuki, A., Sekine, R., Yamamura, M. & Kiga, D., 2014 9月 19, In: ACS Synthetic Biology. 3, 9, p. 638-644 7 p.

研究成果: Article › 査読

6 被引用数 (Scopus)

Multiple amino acid-excluded genetic codes for protein engineering using multiple sets of tRNA variants

Amikura, K., Sakai, Y., Asami, S. & Kiga, D., 2014 3月 21, In: ACS Synthetic Biology. 3, 3, p. 140-144 5 p.

研究成果: Article › 査読

7 被引用数 (Scopus)

Why 20 amino acids?

Amikura, K. & Kiga, D., 2014 6月, In: Kobunshi. 63, 6, p. 392-393 2 p.

研究成果: Article › 査読

2013

Cultivation of synthetic biology with the iGEM competition

Thamamongood, T., Lim, N. Z. L., Ho, T. Y. H., Ayukawa, S., Kiga, D. & Chow, K. L., 2013 3月, In: Journal of Advanced Computational Intelligence and Intelligent Informatics. 17, 2, p. 161-166 6 p.

研究成果: Review article › 査読

Open Access

Synthetic biology and dual use

Kiga, D., 2013, In: Journal of Disaster Research. 8, 4, p. 698-704 7 p.

研究成果: Review article › 査読

The number of amino acids in a genetic code

Amikura, K. & Kiga, D., 2013 8月 21, In: RSC Advances. 3, 31, p. 12512-12517 6 p.

研究成果: Review article › 査読

4 被引用数 (Scopus)

2012

An aptazyme-based molecular device that converts a small-molecule input into an RNA output

Ayukawa, S., Sakai, Y. & Kiga, D., 2012 7月 4, In: Chemical Communications. 48, 61, p. 7556-7558 3 p.

研究成果: Article › 査読

14 被引用数 (Scopus)

Simplification of the genetic code: Restricted diversity of genetically encoded amino acids

Kawahara-Kobayashi, A., Masuda, A., Araiso, Y., Sakai, Y., Kohda, A., Uchiyama, M., Asami, S., Matsuda, T., Ishitani, R., Dohmae, N., Yokoyama, S., Kigawa, T., Nureki, O. & Kiga, D., 2012 11月, In: Nucleic acids research. 40, 20, p. 10576-10584 9 p.

研究成果: Article › 査読

Open Access

17 被引用数 (Scopus)

Tunability of the ratio of cell states after the synthetic diversification by the diversity generator

Sekine, R., Yamamura, M., Hagiya, M. & Kiga, D., 2012, In: Communicative and Integrative Biology. 5, 4, p. 393-394 2 p.

研究成果: Article › 査読

Open Access

2011

RTRACS: A modularized RNA-dependent RNA transcription system with high programmability

Ayukawa, S., Takinoue, M. & Kiga, D., 2011 12月 20, In: Accounts of Chemical Research. 44, 12, p. 1369-1379 11 p.

研究成果: Article › 査読

23 被引用数 (Scopus)

Tunable synthetic phenotypic diversification on Waddington's landscape through autonomous signaling

Sekine, R., Yamamura, M., Ayukawa, S., Ishimatsu, K., Akama, S., Takinoue, M., Hagiya, M. & Kiga, D., 2011 11月 1, In: Proceedings of the National Academy of Sciences of the United States of America. 108, 44, p. 17969-17973 5 p.

研究成果: Article › 査読

Open Access

28 被引用数 (Scopus)

2010

Construction of a genetic AND gate under a new standard for assembly of genetic parts

Ayukawa, S., Kobayashi, A., Nakashima, Y., Takagi, H., Hamada, S., Uchiyama, M., Yugi, K., Murata, S., Sakakibara, Y., Hagiya, M., Yamamura, M. & Kiga, D., 2010 12月 2, In: BMC Genomics. 11, SUPPL. 4, S16.

研究成果: Article › 査読

Open Access

10 被引用数 (Scopus)

2009

Rna oscillator: Limit cycle oscillations based on artificial biomolecular reactions

Takinoue, M., Kiga, D., Shohda, K. I. & Suyama, A., 2009 2月, In: New Generation Computing. 27, 2, p. 107-127 21 p.

研究成果: Article › 査読

8 被引用数 (Scopus)

2008

A design and feasibility study of reactions comprising DNA molecular machine that walks autonomously by using a restriction enzyme

Sekiguchi, H., Komiya, K., Kiga, D. & Yamamura, M., 2008 9月 1, In: Natural Computing. 7, 3, p. 303-315 13 p.

研究成果: Article › 査読

1 被引用数 (Scopus)

A realization of DNA molecular machine that walks autonomously by using a restriction enzyme

Sekiguchi, H., Komiya, K., Kiga, D. & Yamamura, M., 2008 8月 27, DNA Computing - 13th International Meeting on DNA Computing, DNA13, Revised Selected Papers. p. 54-65 12 p. (Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics); vol. 4848 LNCS).

研究成果: Conference contribution

5 被引用数 (Scopus)

Experiments and simulation models of a basic computation element of an autonomous molecular computing system

Takinoue, M., Kiga, D., Shohda, K. I. & Suyama, A., 2008 10月 29, In: Physical Review E - Statistical, Nonlinear, and Soft Matter Physics. 78, 4, 041921.

研究成果: Article › 査読

41 被引用数 (Scopus)

Synthetic biology

Kiga, D. & Yamamura, M., 2008 8月, In: New Generation Computing. 26, 4, p. 347-364 18 p.

研究成果: Article › 査読

2 被引用数 (Scopus)

2007

Oscillator for self-assembled DNA nanomachines

Takinoue, M., Kiga, D., Shohda, K. I. & Suyama, A., 2007 12月 1, p. 104-106. 3 p.

研究成果: Paper › 査読

SYANAC: SYnthetic biological Automaton for Noughts and Crosses

Ayukawa, S., Kobayashi, A., Nakashima, Y., Takagi, H., Hamada, S., Uchiyama, M., Yugi, K., Murata, S., Sakakibara, Y., Hagiya, M., Yamamura, M. & Kiga, D., 2007 8月 15, In: IET Synthetic Biology. 1, 1-2, p. 64-67 4 p.

研究成果: Article › 査読

2 被引用数 (Scopus)

2006

Cation-π interaction in the polyolefin cyclization cascade uncovered by incorporating unnatural amino acids into the catalytic sites of squalene cyclase

Morikubo, N., Fukuda, Y., Ohtake, K., Shinya, N., Kiga, D., Sakamoto, K., Asanuma, M., Hirota, H., Yokoyama, S. & Hoshino, T., 2006 10月 11, In: Journal of the American Chemical Society. 128, 40, p. 13184-13194 11 p.

研究成果: Article › 査読

70 被引用数 (Scopus)

DNA polymerase programmed with a hairpin DNA incorporates a multiple-instruction architecture into molecular computing

Komiya, K., Sakamoto, K., Kameda, A., Yamamoto, M., Ohuchi, A., Kiga, D., Yokoyama, S. & Hagiya, M., 2006 1月, In: BioSystems. 83, 1, p. 18-25 8 p.

研究成果: Article › 査読

22 被引用数 (Scopus)

Translation of 'rare' codons in a cell-free protein synthesis system from Escherichia coli

Chumpolkulwong, N., Sakamoto, K., Hayashi, A., Iraha, F., Shinya, N., Matsuda, N., Kiga, D., Urushibata, A., Shirouzu, M., Oki, K., Kigawa, T. & Yokoyama, S., 2006 3月, In: Journal of structural and functional genomics. 7, 1, p. 31-36 6 p.

研究成果: Article › 査読

25 被引用数 (Scopus)

2002

An engineered Escherichia coli tyrosyl-tRNA synthetase for site-specific incorporation of an unnatural amino acid into proteins in eukaryotic translation and its application in a wheat germ cell-free system

Kiga, D., Sakamoto, K., Kodama, K., Kigawa, T., Matsuda, T., Yabuki, T., Shirouzu, M., Harada, Y., Nakayama, H., Takio, K., Hasegawa, Y., Endo, Y., Hirao, I. & Yokoyama, S., 2002 7月 23, In: Proceedings of the National Academy of Sciences of the United States of America. 99, 15, p. 9715-9720 6 p.

研究成果: Article › 査読

147 被引用数 (Scopus)

Site-specific incorporation of an unnatural amino acid into proteins in mammalian cells

Sakamoto, K., Hayashi, A., Sakamoto, A., Kiga, D., Nakayama, H., Soma, A., Kobayashi, T., Kitabatake, M., Takio, K., Saito, K., Shirouzu, M., Hirao, I. & Yokoyama, S., 2002 11月 1, In: Nucleic acids research. 30, 21, p. 4692-4699 8 p.

研究成果: Article › 査読

Open Access

201 被引用数 (Scopus)

2001

Shifted positioning of the anticodon nucleotide residues of amber suppressor tRNA species by Escherichia coli arginyl-tRNA synthetase

Kiga, D., Sakamoto, K., Sato, S., Hirao, I. & Yokoyama, S., 2001, In: European Journal of Biochemistry. 268, 23, p. 6207-6213 7 p.

研究成果: Article › 査読

Open Access

9 被引用数 (Scopus)

2000

Molecular computation by DNA hairpin formation

Sakamoto, K., Gouzu, H., Komiya, K., Kiga, D., Yokoyama, S., Yokomori, T. & Hagiya, M., 2000 5月 19, In: Science. 288, 5469, p. 1223-1226 4 p.

研究成果: Article › 査読

362 被引用数 (Scopus)

木賀 大介

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木賀大介