大山 隆

研究成果

年別の研究成果 1978 1992 1993 1995 1997 1998 2000 2001 2007 2013 2018 2023

• 67 Article
• 3 Comment/debate
• 2 Review article
• 1 Chapter

年別の研究成果

年別の研究成果

検索結果

• Article

  A common feature shared by bent DNA structures locating in the eukaryotic promoter region

  Miyano, M., Kawashima, T. & Ohyama, T., 2001, In: Molecular Biology Reports. 28, 1, p. 53-61 9 p.

  研究成果: Article › 査読

  • Genetic Promoter Regions 100%
  • DNA 57%
  • Clone Cells 24%
  • Firearms 11%
  • Chromatin 9%
  2 被引用数 (Scopus)

• A designed curved DNA segment that is a remarkable activator of eukaryotic transcription

  Sumida, N., Nishikawa, J. I., Kishi, H., Amano, M., Furuya, T., Sonobe, H. & Ohyama, T., 2006 12月, In: FEBS Journal. 273, 24, p. 5691-5702 12 p.

  研究成果: Article › 査読

  • Thymidine 100%
  • Transgenes 91%
  • Thymidine Kinase 55%
  • COS Cells 52%
  • DNA 50%
  17 被引用数 (Scopus)

• A designed curved DNA sequence remarkably enhances transgene expression from plasmid DNA in mouse liver

  Fukunaga, S., Kanda, G., Tanase, J., Harashima, H., Ohyama, T. & Kamiya, H., 2012 8月, In: Gene Therapy. 19, 8, p. 828-835 8 p.

  研究成果: Article › 査読

  Open Access

  • Transgenes 100%
  • Plasmids 79%
  • DNA 55%
  • Liver 51%
  • Nucleosomes 46%
  12 被引用数 (Scopus)

• Adjacent upstream superhelical writhe influences an Escherichia coli promoter as measured by in Vivo strength and in Vitro open complex formation

  Hirota, Y. & Ohyama, T., 1995 12月 8, In: Journal of Molecular Biology. 254, 4, p. 566-578 13 p.

  研究成果: Article › 査読

  • Escherichia coli 100%
  • DNA 79%
  • Tetracycline Resistance 65%
  • In Vitro Techniques 62%
  • Consensus Sequence 54%
  33 被引用数 (Scopus)

• A highly distinctive mechanical property found in the majority of human promoters and its transcriptional relevance

  Fukue, Y., Sumida, N., Tanase, J. I. & Ohyama, T., 2005, In: Nucleic acids research. 33, 12, p. 3821-3827 7 p.

  研究成果: Article › 査読

  • TATA Box 100%
  • Transcription Initiation Site 60%
  • Statistical Models 29%
  • Genetic Promoter Regions 25%
  • Nucleotides 20%
  33 被引用数 (Scopus)

• Alteration of the curved helical structure located in the upstream region of the β-lactamase promoter of plasmid pUC 19 and its effect on transcription

  Ohyama, T., Nagumo, M., Hirota, Y. & Sakuma, S., 1992 4月 11, In: Nucleic acids research. 20, 7, p. 1617-1622 6 p.

  研究成果: Article › 査読

  • Genetic Promoter Regions 100%
  • Plasmids 83%
  • DNA 57%
  • DNA-Directed RNA Polymerases 57%
  • Oligonucleotides 53%
  22 被引用数 (Scopus)

• A murine Thy-1.2 reporter vector containing a SV40 origin for rapid cloning and analysis of eukaryotic promoters

  Kadokawa, Y., Kusakabe, T., Kamachi, Y., Isobe, K. I., Kondoh, H. & Ohyama, T., 1995 2月 14, In: Gene. 153, 2, p. 277-278 2 p.

  研究成果: Article › 査読

  • COS Cells 100%
  • Organism Cloning 78%
  • Plasmids 46%
  • anti-Thy antibody 43%
  • Fluorescein 30%

• An intrinsic DNA curvature found in the cyanobacterium Microcystis aeruginosa K-81 affects the promoter activity of rpoD1 encoding a principal sigma factor

  Asayama, M., Hayasaka, Y., Kabasawa, M., Shirai, M. & Ohyama, T., 1999 1月 1, In: Journal of biochemistry. 125, 3, p. 460-468 9 p.

  研究成果: Article › 査読

  • Microcystis 100%
  • Sigma Factor 81%
  • Cyanobacteria 79%
  • DNA Structure 72%
  • Tertiary Structure 64%
  22 被引用数 (Scopus)

• An S-like ribonuclease gene is used to generate a trap-leaf enzyme in the carnivorous plant Drosera adelae

  Okabe, T., Iwakiri, Y., Mori, H., Ogawa, T. & Ohyama, T., 2005 10月 24, In: FEBS Letters. 579, 25, p. 5729-5733 5 p.

  研究成果: Article › 査読

  • Drosera 100%
  • Ribonucleases 54%
  • Phosphates 42%
  • Insecta 33%
  • Phosphate 31%
  18 被引用数 (Scopus)

• An ultrarapid method for the recovery of DNA from gels

  Ohyama, T., 1993 1月, In: Analytical Biochemistry. 208, 1, p. 209-211 3 p.

  研究成果: Article › 査読
  2 被引用数 (Scopus)

• A possible function of DNA curvature in transcription.

  Ohyama, T. & Hirota, Y., 1993, In: Nucleic acids symposium series. 29, p. 153-154 2 p.

  研究成果: Article › 査読

  • DNA 100%
  • beta-Lactamases 76%
  • Plasmids 57%
  • Escherichia coli 25%
  • Genes 14%
  2 被引用数 (Scopus)

• A strong structural correlation between short inverted repeat sequences and the polyadenylation signal in yeast and nucleosome exclusion by these inverted repeats

  Miura, O., Ogake, T., Yoneyama, H., Kikuchi, Y. & Ohyama, T., 2019 4月 5, In: Current Genetics. 65, 2, p. 575-590 16 p.

  研究成果: Article › 査読

  Open Access

  • Inverted Repeat Sequences 100%
  • Polyadenylation 83%
  • Initiator Codon 81%
  • Nucleosomes 77%
  • Yeasts 53%
  5 被引用数 (Scopus)

• Bent DNA in the human adenovirus type 2 E1A enhancer is an architectural element for transcription stimulation

  Ohyama, T., 1996, In: Journal of Biological Chemistry. 271, 44, p. 27823-27828 6 p.

  研究成果: Article › 査読

  • Human Adenoviruses 100%
  • Enhancer 89%
  • Nucleic Acid Conformation 55%
  • DNA 42%
  • Genetic Enhancer Elements 24%
  2 被引用数 (Scopus)

• Characterization of DNA fragments that show anomalously rapid migration in non-denaturing polyacrylamide gels.

  Tsujibayashi, H., Tagashira, H. & Ohyama, T., 1997, In: Nucleic acids symposium series. 37, p. 279-280 2 p.

  研究成果: Article › 査読

  • polyacrylamide gels 100%
  • Nucleic Acid Conformation 61%
  • DNA 46%
  • Cattle 33%

• Cloning and characterization of a human genomic sequence that alleviates repeat-induced gene silencing

  Fukuma, M., Ganmyo, Y., Miura, O., Ohyama, T. & Shimizu, N., 2016 4月, In: PloS one. 11, 4, e0153338.

  研究成果: Article › 査読

  Open Access

  • Matrix Attachment Regions 100%
  • gene silencing 68%
  • Gene Silencing 66%
  • Organism Cloning 57%
  • Plasmids 50%
  7 被引用数 (Scopus)

• Competence of an artificial bent DNA as a transcriptional activator in mouse ES cells

  Tanase, J. I., Mitani, T., Udagawa, K., Nishikawa, J. I. & Ohyama, T., 2011 1月, In: Molecular Biology Reports. 38, 1, p. 37-47 11 p.

  研究成果: Article › 査読

  • Mouse Embryonic Stem Cells 100%
  • Mental Competency 78%
  • Cell Line 48%
  • DNA 45%
  • Embryonic Stem Cells 43%
  5 被引用数 (Scopus)

• Conservation of DNA bend sites with identical superhelical twists among the human, mouse, bovine, rabbit and chicken β-globin genes

  Wanapirak, C., Onishi, Y., Wada-Kiyama, Y., Ohyama, T. & Kiyama, R., 2000, In: DNA Research. 7, 4, p. 253-259 7 p.

  研究成果: Article › 査読

  Open Access

  • Globins 100%
  • Chickens 76%
  • Cattle 63%
  • Rabbits 56%
  • DNA 44%
  6 被引用数 (Scopus)

• Core promoter elements of eukaryotic genes have a highly distinctive mechanical property

  Fukue, Y., Sumida, N., Nishikawa, J. I. & Ohyama, T., 2004, In: Nucleic acids research. 32, 19, p. 5834-5840 7 p.

  研究成果: Article › 査読

  • TATA Box 100%
  • TATA-Box Binding Protein 37%
  • Genes 31%
  • Adenine 30%
  • RNA Polymerase II 30%
  33 被引用数 (Scopus)

• Cruciform formable sequences within pou5f1 enhancer are indispensable for mouse es cell integrity

  Yamamoto, Y., Miura, O. & Ohyama, T., 2021 4月 1, In: International journal of molecular sciences. 22, 7, 3399.

  研究成果: Article › 査読

  Open Access

  • genome 100%
  • Enhancer 91%
  • Transcription 89%
  • Untranslated RNA 86%
  • integrity 81%
  3 被引用数 (Scopus)

• Deoxyribonuclease secreted from an insectivorous plant Drosera adelae.

  Okabe, T., Mori, H. & Ohyama, T., 1997, In: Nucleic acids symposium series. 37, p. 127-128 2 p.

  研究成果: Article › 査読

  • Drosera 100%
  • Deoxyribonucleases 60%
  • Enzymes 26%
  • Divalent Cations 19%
  • Endonucleases 19%
  2 被引用数 (Scopus)

• DNA conformation of the region preceding the beta-lactamase promoter of pUC19 which is required for efficient transcription.

  Hirota, Y. & Ohyama, T., 1992, In: Nucleic acids symposium series. 27, p. 151-152 2 p.

  研究成果: Article › 査読

  • Nucleic Acid Conformation 100%
  • beta-Lactamases 72%
  • Plasmids 54%
  • Ampicillin Resistance 32%
  • Escherichia coli 31%
  1 被引用数 (Scopus)

• Effects of a conformational change in tRNA on its aminoacylation capacity: a study using enzymatically reconstructed variants of T. utilis tRNATyr.

  Ohyama, T., Nishikawa, K. & Takemura, S., 1985 12月 1, In: Nucleic acids symposium series. 16, p. 213-215 3 p.

  研究成果: Article › 査読

  • RNA, Transfer, Tyr 100%
  • Aminoacylation 85%
  • Transfer RNA 64%
  • Ribonuclease T1 46%
  • Conserved Sequence 35%

• Effects of an upstream DNA curvature on the strength of HSV thymidine kinase promoter.

  Ohyama, T., Kadokawa, Y. & Hirota, Y., 1995, In: Nucleic acids symposium series. 34, p. 67-68 2 p.

  研究成果: Article › 査読

  • Thymidine Kinase 100%
  • DNA 45%
  • Simplexvirus 44%
  • Oligonucleotides 42%
  • Genetic Promoter Regions 39%

• Effects of carriers on transgene expression from plasmids containing a DNA sequence with high histone affinity

  Kamiya, H., Fukunaga, S., Ohyama, T. & Harashima, H., 2009 7月 6, In: International Journal of Pharmaceutics. 376, 1-2, p. 99-103 5 p.

  研究成果: Article › 査読

  • Histone 100%
  • DNA Sequence 96%
  • Transgenes 94%
  • Histones 87%
  • Plasmids 75%
  3 被引用数 (Scopus)

• Enzymatic replacement of nucleotide sequences in the D-loop of T. utilis tRNATyr and its effect on aminoacylation.

  Ohyama, T., Nishikawa, K. & Takemura, S., 1983 12月 1, In: Nucleic acids symposium series. 12, p. 137-140 4 p.

  研究成果: Article › 査読

  • RNA, Transfer, Tyr 100%
  • Aminoacylation 85%
  • Transfer RNA 21%
  • Tyrosine 18%
  2 被引用数 (Scopus)

• Expression and function of an S1-type nuclease in the digestive fluid of a sundew, Drosera adelae

  Yu, M., Arai, N., Ochiai, T. & Ohyama, T., 2023 3月 8, In: Annals of Botany. 131, 2, p. 335-346 12 p.

  研究成果: Article › 査読

  • Drosera adelae 100%
  • Drosera 95%
  • nucleases 49%
  • fluids 34%
  • carnivorous plants 26%

• Functional analyses of carnivorous plant-specific amino acid residues in S-like ribonucleases

  Arai, N., Nishimura, E., Kikuchi, Y. & Ohyama, T., 2015 7月 30, In: Biochemical and Biophysical Research Communications. 465, 1, p. 108-112 5 p., 34351.

  研究成果: Article › 査読

  • Ribonucleases 100%
  • Carnivory 96%
  • Amino-Acid Residue 79%
  • Amino Acids 62%
  • Mutation 56%
  3 被引用数 (Scopus)

• Functional significance of bent DNA in transcriptional regulation

  Ohyama, T., 1990 3月, In: Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme. 35, 3, p. 248-251 4 p.

  研究成果: Article › 査読

• Functional significance of the DNA curvature located near a promoter: an analysis using the beta-lactamase promoter of pUC19.

  Hirota, Y. & Ohyama, T., 1991, In: Nucleic acids symposium series. 25, p. 117-118 2 p.

  研究成果: Article › 査読

  • beta-Lactamases 100%
  • DNA 52%
  • Genetic Promoter Regions 45%
  • Genes 18%

• Genetic information carried in DNA conformation and physical properties

  Ohyama, T., 2008 1月 1, In: Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme. 53, 1, p. 1-11 11 p.

  研究成果: Article › 査読

• High-efficiency shotgun cloning of curved DNA segments from chromosomal DNA

  Ohyama, T. & Kusakabe, T., 1993 7月, In: Analytical Biochemistry. 212, 1, p. 287-289 3 p.

  研究成果: Article › 査読
  6 被引用数 (Scopus)

• Highly efficient chromatin transcription induced by superhelically curved DNA segments: The underlying mechanism revealed by a yeast system

  Tanase, J. I., Morohashi, N., Fujita, M., Nishikawa, J. I., Shimizu, M. & Ohyama, T., 2010 3月 23, In: Biochemistry. 49, 11, p. 2351-2358 8 p.

  研究成果: Article › 査読

  • DNA Structure 100%
  • Nucleosomes 99%
  • Chromatin 74%
  • Yeasts 68%
  • DNA 47%
  8 被引用数 (Scopus)

• Improvement of the aluminum borate whisker-mediated method of DNA delivery into rice callus

  Mizuno, K., Takahashi, W., Ohyama, T., Shimada, T. & Tanaka, O., 2004 3月, In: Plant Production Science. 7, 1, p. 45-49 5 p.

  研究成果: Article › 査読

  • borates 100%
  • agitation 84%
  • aluminum 71%
  • callus 68%
  • rice 51%
  16 被引用数 (Scopus)

• Influence of highly curved DNA segments on in vivo topology of plasmids

  Ohyama, T., Miyano, M. & Sakuma, S., 1999 12月, In: Molecular Biology Reports. 26, 4, p. 269-276 8 p.

  研究成果: Article › 査読

  • Plasmids 100%
  • DNA 69%
  • DNA Topoisomerases 29%
  • Escherichia coli 24%
  • Human Adenoviruses 23%
  1 被引用数 (Scopus)

• In vivo gene transfer to mouse spermatogenic cells by deoxyribonucleic acid injection into seminiferous tubules and subsequent electroporation

  Yamazaki, Y., Fujimoto, H., Ando, H., Ohyama, T., Hirota, Y. & Noce, T., 1998 12月, In: Biology of Reproduction. 59, 6, p. 1439-1444 6 p.

  研究成果: Article › 査読

  • Protamines 100%
  • Seminiferous Tubules 98%
  • Electroporation 94%
  • Transgenes 53%
  • Testis 46%
  98 被引用数 (Scopus)

• Involvement of the response regulator CtrA in the extracellular DNA production of the marine bacterium Rhodovulum sulfidophilum

  Komatsu, H., Yamamoto, J., Suzuki, H., Nagao, N., Hirose, Y., Ohyama, T., Umekage, S. & Kikuchi, Y., 2018, In: Journal of General and Applied Microbiology. 64, 3, p. 103-107 5 p.

  研究成果: Article › 査読

  • Rhodovulum 100%
  • Bacteria 32%
  • DNA 27%
  • Nucleic Acids 15%
  • Culture Media 14%
  3 被引用数 (Scopus)

• Isolation of replicational cue elements from a library of bent DNAs of Aspergillus oryzae

  Kusakabe, T., Sugimoto, Y., Hirota, Y., Toné, S., Kawaguchi, Y., Koga, K. & Ohyama, T., 2000, In: Molecular Biology Reports. 27, 1, p. 13-19 7 p.

  研究成果: Article › 査読

  • Aspergillus oryzae 100%
  • Gene Library 67%
  • Cues 55%
  • Aspergillus nidulans 44%
  • Type II DNA Topoisomerase 41%
  2 被引用数 (Scopus)

• Left-handedly curved DNA regulates accessibility to cis-DNA elements in chromatin

  Nishikawa, J. I., Amano, M., Fukue, Y., Tanaka, S., Kishi, H., Hirota, Y., Yoda, K. & Ohyama, T., 2003 11月 15, In: Nucleic acids research. 31, 22, p. 6651-6662 12 p.

  研究成果: Article › 査読

  • Chromatin 100%
  • DNA 63%
  • Nucleosomes 58%
  • TATA Box 16%
  • Trans-Activators 15%
  28 被引用数 (Scopus)

• Linearization and integration of DNA into cells preferentially occurs at intrinsically curved regions from human LINE-1 repetitive element

  Kusakabe, T., Sugimoto, Y., Maeda, T., Nakajima, Y., Miyano, M., Nishikawa, J. I., Tone, S., Kawaguchi, Y., Koga, K. & Ohyama, T., 2001 8月 22, In: Gene. 274, 1-2, p. 271-281 11 p.

  研究成果: Article › 査読

  • Long Interspersed Nucleotide Elements 100%
  • Clone Cells 41%
  • DNA 36%
  • Human Chromosomes 29%
  • Chromosome Deletion 16%
  3 被引用数 (Scopus)

• Magnesium chloride and polyamine can differentiate mouse embryonic stem cells into trophectoderm or endoderm

  Tanase, J. I., Yokoo, T., Matsumura, Y., Kinoshita, M., Kikuchi, Y., Suemori, H. & Ohyama, T., 2017 1月 22, In: Biochemical and Biophysical Research Communications. 482, 4, p. 764-770 7 p.

  研究成果: Article › 査読

  • Magnesium Chloride 100%
  • Endoderm 94%
  • Mouse Embryonic Stem Cells 92%
  • Polyamines 88%
  • Polyamine 80%
  2 被引用数 (Scopus)

• Mechanism of transcriptional regulation by bent DNA structures

  Ohyama, T., 1998 12月 1, In: Seikagaku. 70, 11, p. 1339-1344 6 p.

  研究成果: Article › 査読

• Most methylation-susceptible DNA sequences in human embryonic stem cells undergo a change in conformation or flexibility upon methylation

  Shimooka, Y., Nishikawa, J. I. & Ohyama, T., 2013 2月 26, In: Biochemistry. 52, 8, p. 1344-1353 10 p.

  研究成果: Article › 査読

  • Human Embryonic Stem Cells 100%
  • DNA Sequence 85%
  • Methylation 75%
  • Flexibility 65%
  • DNA Methylation 40%
  12 被引用数 (Scopus)

• Multimerization of restriction fragments by magnesium-mediated stable base pairing between overhangs: A cause of electrophoretic mobility shift

  Tagashira, H., Morita, M. & Ohyama, T., 2002 10月 8, In: Biochemistry. 41, 40, p. 12217-12223 7 p.

  研究成果: Article › 査読

  • Electrophoretic Mobility Shift Assay 100%
  • Electrophoretic Mobility 96%
  • Base Pairing 93%
  • Magnesium 92%
  • Compound Mobility 69%
  4 被引用数 (Scopus)

• Nuclear localization of reporter genes activated by curved DNA

  Udagawa, K., Kimura, H., Tanabe, H. & Ohyama, T., 2012 4月, In: Journal of Bioscience and Bioengineering. 113, 4, p. 431-437 7 p.

  研究成果: Article › 査読

  • Electroporation 100%
  • DNA Structure 84%
  • Histone 76%
  • DNA 73%
  • Reporter Genes 70%
  1 被引用数 (Scopus)

• Organ-specific expression and epigenetic traits of genes encoding digestive enzymes in the lance-leaf sundew (Drosera adelae)

  Arai, N., Ohno, Y., Jumyo, S., Hamaji, Y. & Ohyama, T., 2021 2月 27, In: Journal of Experimental Botany. 72, 5, p. 1946-1961 16 p.

  研究成果: Article › 査読

  Open Access

  • Drosera adelae 100%
  • Drosera 95%
  • digestive enzymes 52%
  • epigenetics 45%
  • Epigenomics 40%
  4 被引用数 (Scopus)

• Peculiar transition observed on the temperature dependence of water permeability of a novel hydrophilic polyamide membrane

  Sumitomo, H., Hashimoto, K. & Ohyama, T., 1979 8月 1, In: Polymer Bulletin. 1, 9, p. 635-639 5 p.

  研究成果: Article › 査読

  • Polyamides 100%
  • Polyamide 80%
  • permeability 73%
  • Membranes 70%
  • Permeability 66%
  1 被引用数 (Scopus)

• Positions of pluripotency genes and hepatocyte-specific genes in the nucleus before and after mouse ES cell differentiation

  Udagawa, K. & Ohyama, T., 2014 3月 24, In: Genetics and Molecular Research. 13, 1, p. 1979-1988 10 p.

  研究成果: Article › 査読

  • Gene Order 100%
  • Mouse Embryonic Stem Cells 95%
  • Hepatocytes 64%
  • Cell Differentiation 62%
  • Genes 31%
  2 被引用数 (Scopus)

• Quantitation of mRNA using in vitro RNA amplification and Northern hybridization

  Toné, S., Kubo, T., Ohyama, T., Kusakabe, T. & Minatogawa, Y., 2000 9月 10, In: Analytical Biochemistry. 284, 2, p. 420-422 3 p.

  研究成果: Article › 査読

• Regions of unusually high flexibility occur frequently in human genomic DNA

  Kimura, H., Kageyama, D., Furuya, M., Sugiyama, S., Murata, N. & Ohyama, T., 2013, In: Bioscience, Biotechnology and Biochemistry. 77, 3, p. 612-617 6 p.

  研究成果: Article › 査読

  • DNA Sequence 100%
  • Flexibility 76%
  • Chromosomes 76%
  • DNA 53%
  • Diptera 52%
  1 被引用数 (Scopus)

• Requirement or exclusion of inverted repeat sequences with cruciform-forming potential in Escherichia coli revealed by genome-wide analyses

  Miura, O., Ogake, T. & Ohyama, T., 2018 8月 1, In: Current Genetics. 64, 4, p. 945-958 14 p.

  研究成果: Article › 査読

  Open Access

  • Inverted Repeat Sequences 100%
  • Initiator Codon 81%
  • Terminator Codon 59%
  • Escherichia coli 46%
  • Genome 44%
  9 被引用数 (Scopus)