抄録
The helical filament formed by RecA protein on single-stranded DNA plays an important role in homologous recombination and pairs with a complementary single strand or homologous duplex DNA. The RecA nucleoprotein filament also recognizes an identical single strand. The chimeric protein, RecAc38, forms a nucleoprotein filament that recognizes a complementary strand but is defective in recognition of duplex DNA, and is associated with phenotypic defects in repair and recombination. As described here, RecAc38 nucleoprotein filament is also defective in recognition of an identical strand, either when the filament has within it a single strand or duplex DNA. A model that postulates three DNA binding sites rationalizes these observations and suggests that the third binding site mediates non-Watson-Crick interactions that are instrumental in recognition of homology in duplex DNA.
| 本文言語 | English |
|---|---|
| ページ（範囲） | 3387-3391 |
| ページ数 | 5 |
| ジャーナル | Nucleic Acids Research |
| 巻 | 22 |
| 号 | 16 |
| 出版ステータス | Published - 1994 8月 25 |
| 外部発表 | はい |
ASJC Scopus subject areas
- 遺伝学
- 統計学、確率および不確実性
- 応用数学
- 健康、毒物学および変異誘発
- 毒物学
- 遺伝学（臨床）