A Cu-free clickable fluorescent probe for intracellular targeting of small biomolecules

Kento Yamagishi; Kazuaki Sawaki; Atsushi Murata; Shinji Takeoka

doi:10.1039/c4cc09947h

A Cu-free clickable fluorescent probe for intracellular targeting of small biomolecules

Kento Yamagishi, Kazuaki Sawaki, Atsushi Murata, Shinji Takeoka^*

^*この研究の対応する著者

先進理工学部

研究成果: Article › 査読

13 被引用数 (Scopus)

抄録

We synthesized a novel cyclooctyne-based clickable fluorescent probe with versatile properties such as high cell-membrane permeability and free diffusibility in the cell. Our probe "FC-DBCO" was conjugated to an azide-modified mannose via a Cu-free click reaction in living HeLa cells and displayed intracellular specific fluorescence imaging with low background signals.

本文言語	English
ページ（範囲）	7879-7882
ページ数	4
ジャーナル	Chemical Communications
巻	51
号	37
DOI	https://doi.org/10.1039/c4cc09947h
出版ステータス	Published - 2015 5月 7

ASJC Scopus subject areas

触媒
電子材料、光学材料、および磁性材料
セラミックおよび複合材料
化学 (全般)
表面、皮膜および薄膜
金属および合金
材料化学

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AU - Sawaki, Kazuaki

AU - Murata, Atsushi

AU - Takeoka, Shinji

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A Cu-free clickable fluorescent probe for intracellular targeting of small biomolecules

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