A microfluidic mammalian cell sorter with thermal gelation polymer solution

Y. Shirasaki, M. Goto, H. Sugino, T. Arakawa, D. Yoon, J. Mizuno, S. Shoji, T. Funatsu, O. Ohara*

*この研究の対応する著者

研究成果: Conference contribution

2 被引用数 (Scopus)

抄録

We report a development of an on-chip sorting system for fluorescently labeled mammalian cells based on a reversible sol-gel transition of thermoreversible gelation polymer controlled by local heating with infrared laser. The new system allowed to utilize the energy from the infrared laser irradiation efficiently to achieve an extensive gelation of the polymer in the channel. This enabled the new system to sort mammalian cells with 97% viability in the large channel with 50-100 μm in depth and width. Moreover, we succeeded in sorting with 100% recovery and over 99% purity by introducing a 3D flow focusing.

本文言語English
ホスト出版物のタイトル14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
ページ1571-1573
ページ数3
出版ステータスPublished - 2010
外部発表はい
イベント14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 - Groningen, Netherlands
継続期間: 2010 10月 32010 10月 7

出版物シリーズ

名前14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
3

Conference

Conference14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
国/地域Netherlands
CityGroningen
Period10/10/310/10/7

ASJC Scopus subject areas

  • 制御およびシステム工学

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