A novel carotenoid biosynthetic route via oxidosqualene

Yusuke Otani; Takashi Maoka; Shigeko Kawai-Noma; Kyoichi Saito; Daisuke Umeno

doi:10.1016/j.bbrc.2022.01.105

A novel carotenoid biosynthetic route via oxidosqualene

Yusuke Otani, Takashi Maoka, Shigeko Kawai-Noma, Kyoichi Saito, Daisuke Umeno^*

^*この研究の対応する著者

先進理工学部

研究成果: Article › 査読

抄録

Over 800 known carotenoids are synthesized from phytoene or 4,4′-diapophytoene (dehydrosqualene) characterized by three conjugated double bonds. In this paper, we report that carotenoid desaturase CrtN from Staphylococcus aureus and Methylomonas can accept oxidosqualene, which is the precursor for plant- or animal-type triterpenoids, yielding the yellow carotenoid pigments with 8, 9, or 10 conjugated double bonds. The resulting pathway is the second nonnatural route for carotenoid pigments and the first pathway for carotenoid pigments not biosynthesized via (diapo)phytoene.

本文言語	English
ページ（範囲）	75-80
ページ数	6
ジャーナル	Biochemical and Biophysical Research Communications
巻	599
DOI	https://doi.org/10.1016/j.bbrc.2022.01.105
出版ステータス	Published - 2022 4月 9

ASJC Scopus subject areas

生物理学
生化学
分子生物学
細胞生物学

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10.1016/j.bbrc.2022.01.105

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title = "A novel carotenoid biosynthetic route via oxidosqualene",

abstract = "Over 800 known carotenoids are synthesized from phytoene or 4,4′-diapophytoene (dehydrosqualene) characterized by three conjugated double bonds. In this paper, we report that carotenoid desaturase CrtN from Staphylococcus aureus and Methylomonas can accept oxidosqualene, which is the precursor for plant- or animal-type triterpenoids, yielding the yellow carotenoid pigments with 8, 9, or 10 conjugated double bonds. The resulting pathway is the second nonnatural route for carotenoid pigments and the first pathway for carotenoid pigments not biosynthesized via (diapo)phytoene.",

keywords = "Carotenoid, Non-natural routes, Oxidosqualene, Squalene, Triterpenoid",

author = "Yusuke Otani and Takashi Maoka and Shigeko Kawai-Noma and Kyoichi Saito and Daisuke Umeno",

note = "Funding Information: This work was supported by Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology [JSPS Kakenhi-Grants 16H06450 , 18H01791 , 21H01721 ] and by JSPS KAKENHI Grant Number 20J11505 Foundation to Y.O. Publisher Copyright: {\textcopyright} 2022 Elsevier Inc.",

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doi = "10.1016/j.bbrc.2022.01.105",

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AU - Otani, Yusuke

AU - Maoka, Takashi

AU - Kawai-Noma, Shigeko

AU - Saito, Kyoichi

AU - Umeno, Daisuke

N1 - Funding Information: This work was supported by Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology [JSPS Kakenhi-Grants 16H06450 , 18H01791 , 21H01721 ] and by JSPS KAKENHI Grant Number 20J11505 Foundation to Y.O. Publisher Copyright: © 2022 Elsevier Inc.

PY - 2022/4/9

Y1 - 2022/4/9

N2 - Over 800 known carotenoids are synthesized from phytoene or 4,4′-diapophytoene (dehydrosqualene) characterized by three conjugated double bonds. In this paper, we report that carotenoid desaturase CrtN from Staphylococcus aureus and Methylomonas can accept oxidosqualene, which is the precursor for plant- or animal-type triterpenoids, yielding the yellow carotenoid pigments with 8, 9, or 10 conjugated double bonds. The resulting pathway is the second nonnatural route for carotenoid pigments and the first pathway for carotenoid pigments not biosynthesized via (diapo)phytoene.

AB - Over 800 known carotenoids are synthesized from phytoene or 4,4′-diapophytoene (dehydrosqualene) characterized by three conjugated double bonds. In this paper, we report that carotenoid desaturase CrtN from Staphylococcus aureus and Methylomonas can accept oxidosqualene, which is the precursor for plant- or animal-type triterpenoids, yielding the yellow carotenoid pigments with 8, 9, or 10 conjugated double bonds. The resulting pathway is the second nonnatural route for carotenoid pigments and the first pathway for carotenoid pigments not biosynthesized via (diapo)phytoene.

KW - Carotenoid

KW - Non-natural routes

KW - Oxidosqualene

KW - Squalene

KW - Triterpenoid

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JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

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A novel carotenoid biosynthetic route via oxidosqualene

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