TY - JOUR
T1 - A novel cloverleaf structure found in mammalian mitochondrial tRNASer (UCN)
AU - Yokogawa, Takashi
AU - Watanabe, Yoh ichi
AU - Kumazawa, Yoshinori
AU - Ueda, Takuya
AU - Hirao, Ichiro
AU - Miura, Kin ichiro
AU - Watanabe, Kimitsuna
N1 - Funding Information:
We are indebted to Mr. J. Moriya for his technical assistance, and to Dr. K. Nishikawa and Mr. S. Yoshinari for their many pieces of useful advice on the RNA sequencing and the enzymatic probing. This work was supported by a Grant-in-Aid for Scientific Research on Priority Areas from the Ministry of Education, Science and Culture of Japan.
PY - 1991/11/25
Y1 - 1991/11/25
N2 - Bovine mitochondrial tRNASer(UCN) has been thought to have two U-U mismatches at the top of the acceptor stem, as inferred from its gene sequence. However, this unusual structure has not been confirmed at the RNA level. In the course of investigating the structure and function of mitochondrial tRNAs, we have isolated thebovine liver mitochondrial tRNASer(UCN) and determined its complete sequence including the modified nucleotides. Analysis of the 5′-terminal nucleotide and enzymatic determination of the whole sequence of tRNASer(UCN) revealed that the tRNA started from the third nucleotide of the putative tRNASer(UCN) gene, which had formerly been supposed. Enzymatic probing of tRNASer(UCN) suggests that the tRNA possesses an unusual cloverleaf structure with the following characteristics. (1) There exists only one nucleotide between the acceptor stem with 7 base pairs and the D stem with 4 base pairs. (2) The anticodon stem seems to consist of 6 base pairs. Since the same type of cloverleaf structure as above could be constructed only for mitochondrial tRNASer(UCN) genes of mammals such as human, rat and mouse, but not for those of nonmammals such as chicken and frog, this unusual secondary structure seems to be conserved only in mammalian mitochondria.
AB - Bovine mitochondrial tRNASer(UCN) has been thought to have two U-U mismatches at the top of the acceptor stem, as inferred from its gene sequence. However, this unusual structure has not been confirmed at the RNA level. In the course of investigating the structure and function of mitochondrial tRNAs, we have isolated thebovine liver mitochondrial tRNASer(UCN) and determined its complete sequence including the modified nucleotides. Analysis of the 5′-terminal nucleotide and enzymatic determination of the whole sequence of tRNASer(UCN) revealed that the tRNA started from the third nucleotide of the putative tRNASer(UCN) gene, which had formerly been supposed. Enzymatic probing of tRNASer(UCN) suggests that the tRNA possesses an unusual cloverleaf structure with the following characteristics. (1) There exists only one nucleotide between the acceptor stem with 7 base pairs and the D stem with 4 base pairs. (2) The anticodon stem seems to consist of 6 base pairs. Since the same type of cloverleaf structure as above could be constructed only for mitochondrial tRNASer(UCN) genes of mammals such as human, rat and mouse, but not for those of nonmammals such as chicken and frog, this unusual secondary structure seems to be conserved only in mammalian mitochondria.
UR - http://www.scopus.com/inward/record.url?scp=0026014994&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026014994&partnerID=8YFLogxK
U2 - 10.1093/nar/19.22.6101
DO - 10.1093/nar/19.22.6101
M3 - Article
C2 - 1840673
AN - SCOPUS:0026014994
SN - 0305-1048
VL - 19
SP - 6101
EP - 6105
JO - Nucleic acids research
JF - Nucleic acids research
IS - 22
ER -