A novel method of cultivating cardiac myocytes in agarose microchamber chips for studying cell synchronization

Kensuke Kojima, Tomoyuki Kaneko, Kenji Yasuda*

*この研究の対応する著者

研究成果: Article査読

19 被引用数 (Scopus)

抄録

We have developed a new method that enables agar microstructures to be used to cultivate cardiac myocyte cells in a manner that allows their connection patterns to be controlled. Non-contact three-dimensional photo-thermal etching with a 1064-nm infrared focused laser beam was used to form the shapes of agar microstructures. This wavelength was selected as it is not absorbed by water or agar. Identical rat cardiac myocytes were cultured in adjacent microstructures connected by microchannels and the interactions of asynchronous beating cardiac myocyte cells observed. Two isolated and independently beating cardiac myocytes were shown to form contacts through the narrow microchannels and by 90 minutes had synchronized their oscillations. This occurred by one of the two cells stopping their oscillation and following the pattern of the other cell. In contrast, when two sets of synchronized beating cells came into contact, those two sets synchronized without any observable interruptions to their rhythms. The results indicate that the synchronization process of cardiac myocytes may be dependent on the community size and network pattern of these cells.

本文言語English
論文番号9
ジャーナルJournal of Nanobiotechnology
2
DOI
出版ステータスPublished - 2004 9月 9
外部発表はい

ASJC Scopus subject areas

  • バイオエンジニアリング
  • 医学(その他)
  • 分子医療
  • 生体医工学
  • 応用微生物学とバイオテクノロジー
  • 薬科学

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