抄録
We have developed a simplified method for the preparation of liver nuclear extracts to study gene regulation and protein-DNA interactions. This protocol uses conventional laboratory equipment and standard reagents. The liver tissue is homogenized in a low-salt solution at physiological molarity with subsequent adjustment of the molarity and purification of nuclei by density sedimentation. The nuclear extracts are transcriptionally active in a validated cell-free transcription assay and contain functional DNA-binding proteins. This protocol results in the rapid preparation of highly reproducible and active liver nuclear extracts.
| 本文言語 | English |
|---|---|
| ページ（範囲） | 777-781 |
| ページ数 | 5 |
| ジャーナル | DNA and Cell Biology |
| 巻 | 9 |
| 号 | 10 |
| 出版ステータス | Published - 1990 |
| 外部発表 | はい |
ASJC Scopus subject areas
- 分子生物学
- 遺伝学
- 細胞生物学