TY - JOUR
T1 - AAU-specific RNA cleavage mediated by MazF toxin endoribonuclease conserved in Nitrosomonas Europaea
AU - Miyamoto, Tatsuki
AU - Yokota, Akiko
AU - Tsuneda, Satoshi
AU - Noda, Naohiro
N1 - Publisher Copyright:
© 2016 by the authors; licensee MDPI, Basel, Switzerland.
PY - 2016/6
Y1 - 2016/6
N2 - Nitrosomonas europaea carries numerous toxin-antitoxin systems. However, despite the abundant representation in its chromosome, studies have not surveyed the underlying molecular functions in detail, and their biological roles remain enigmatic. In the present study, we found that a chromosomally-encoded MazF family member, predicted at the locus NE1181, is a functional toxin endoribonuclease, and constitutes a toxin-antitoxin system, together with its cognate antitoxin, MazE. Massive parallel sequencing provided strong evidence that this toxin endoribonuclease exhibits RNA cleavage activity, primarily against the AAU triplet. This sequence-specificity was supported by the results of fluorometric assays. Our results indicate that N. europaea alters the translation profile and regulates its growth using the MazF family of endoribonuclease under certain stressful conditions.
AB - Nitrosomonas europaea carries numerous toxin-antitoxin systems. However, despite the abundant representation in its chromosome, studies have not surveyed the underlying molecular functions in detail, and their biological roles remain enigmatic. In the present study, we found that a chromosomally-encoded MazF family member, predicted at the locus NE1181, is a functional toxin endoribonuclease, and constitutes a toxin-antitoxin system, together with its cognate antitoxin, MazE. Massive parallel sequencing provided strong evidence that this toxin endoribonuclease exhibits RNA cleavage activity, primarily against the AAU triplet. This sequence-specificity was supported by the results of fluorometric assays. Our results indicate that N. europaea alters the translation profile and regulates its growth using the MazF family of endoribonuclease under certain stressful conditions.
KW - MazF
KW - Nitrosomonas europaea
KW - Sequence-specific endoribonuclease
KW - Toxin-antitoxin system
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U2 - 10.3390/toxins8060174
DO - 10.3390/toxins8060174
M3 - Article
C2 - 27271670
AN - SCOPUS:84973442056
SN - 2072-6651
VL - 8
JO - Toxins
JF - Toxins
IS - 6
M1 - 174
ER -