TY - JOUR
T1 - Activation of macrophages by a laccase-polymerized polyphenol is dependent on phosphorylation of Rac1
AU - Tajima, Katsuya
AU - Akanuma, Satoshi
AU - Matsumoto-Akanuma, Akiko
AU - Yamanaka, Daisuke
AU - Ishibashi, Ken ichi
AU - Adachi, Yoshiyuki
AU - Ohno, Naohito
N1 - Funding Information:
This work was supported by MEXT-Supported Program for the Strategic Research Foundation at Private Universities , S1512002 , 2015–2017.
Publisher Copyright:
© 2017 Elsevier Inc.
PY - 2018/1/15
Y1 - 2018/1/15
N2 - Various physiologically active effects of polymerized polyphenols have been reported. In this study, we synthesized a polymerized polyphenol (mL2a-pCA) by polymerizing caffeic acid using mutant Agaricus brasiliensis laccase and analyzed its physiological activity and mechanism of action. We found that mL2a-pCA induced morphological changes and the production of cytokines and chemokines in C3H/HeN mouse-derived resident peritoneal macrophages in vitro. The mechanisms of action of polymerized polyphenols on in vitro mouse resident peritoneal cells have not been characterized in detail previously. Herein, we report that the mL2a-pCA-induced production of interleukin-6 (IL-6) and monocyte chemotactic protein-1 (MCP-1) in C3H/HeN mouse-derived resident peritoneal cells was inhibited by treatment with the Rac1 inhibitor NSC23766 trihydrochloride. In addition, we found that mL2a-pCA activated the phosphorylation Rac1. Taken together, the results show that mL2a-pCA induced macrophage activation via Rac1 phosphorylation-dependent pathways.
AB - Various physiologically active effects of polymerized polyphenols have been reported. In this study, we synthesized a polymerized polyphenol (mL2a-pCA) by polymerizing caffeic acid using mutant Agaricus brasiliensis laccase and analyzed its physiological activity and mechanism of action. We found that mL2a-pCA induced morphological changes and the production of cytokines and chemokines in C3H/HeN mouse-derived resident peritoneal macrophages in vitro. The mechanisms of action of polymerized polyphenols on in vitro mouse resident peritoneal cells have not been characterized in detail previously. Herein, we report that the mL2a-pCA-induced production of interleukin-6 (IL-6) and monocyte chemotactic protein-1 (MCP-1) in C3H/HeN mouse-derived resident peritoneal cells was inhibited by treatment with the Rac1 inhibitor NSC23766 trihydrochloride. In addition, we found that mL2a-pCA activated the phosphorylation Rac1. Taken together, the results show that mL2a-pCA induced macrophage activation via Rac1 phosphorylation-dependent pathways.
KW - Agaricus brasiliensis
KW - Laccase
KW - Macrophage
KW - Polymerized polyphenol
KW - Rac1
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U2 - 10.1016/j.bbrc.2017.12.095
DO - 10.1016/j.bbrc.2017.12.095
M3 - Article
C2 - 29269293
AN - SCOPUS:85038830756
SN - 0006-291X
VL - 495
SP - 2209
EP - 2213
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -