TY - JOUR
T1 - Additive effect of mPer1 and mPer2 antisense oligonucleotides on light-induced phase shift
AU - Wakamatsu, Hisanori
AU - Takahashi, Satomi
AU - Moriya, Takahiro
AU - Inouye, Shin Ichi T.
AU - Okamura, Hitoshi
AU - Akiyama, Masashi
AU - Shibata, Shigenobu
PY - 2001/1/22
Y1 - 2001/1/22
N2 - It is well known that light induces both mPer1 and mPer2 mRNA in the suprachiasmatic nucleus. We have reported that mPer1 antisense oligonucleotides (ODNs) inhibited the light-induced phase delays of mouse locomotor rhythm. In this study, we asked whether both or either mPer1 or mPer2 expression is necessary to induce the phase shift. We examined the effects of inhibition of mRNA expression on light-induced phase delays of mouse circadian behavior rhythm. Light-induced phase delays were moderately attenuated by microinjection of mPer1 or mPer2 antisense ODN, but not by mPer3 antisense or mPer1, mPer2 scrambled ODNs, whereas following simultaneous injection of both mPer1 and mPer2 antisense ODNs they disappeared. The present results suggest that acute induction of mPer1 and mPer2 gene play an additive effect on photic entrainment.
AB - It is well known that light induces both mPer1 and mPer2 mRNA in the suprachiasmatic nucleus. We have reported that mPer1 antisense oligonucleotides (ODNs) inhibited the light-induced phase delays of mouse locomotor rhythm. In this study, we asked whether both or either mPer1 or mPer2 expression is necessary to induce the phase shift. We examined the effects of inhibition of mRNA expression on light-induced phase delays of mouse circadian behavior rhythm. Light-induced phase delays were moderately attenuated by microinjection of mPer1 or mPer2 antisense ODN, but not by mPer3 antisense or mPer1, mPer2 scrambled ODNs, whereas following simultaneous injection of both mPer1 and mPer2 antisense ODNs they disappeared. The present results suggest that acute induction of mPer1 and mPer2 gene play an additive effect on photic entrainment.
KW - Antisense oligonucleotides
KW - Circadian clock
KW - MPer
KW - Suprachiasmatic nucleus
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U2 - 10.1097/00001756-200101220-00033
DO - 10.1097/00001756-200101220-00033
M3 - Article
C2 - 11201072
AN - SCOPUS:0035931433
SN - 0959-4965
VL - 12
SP - 127
EP - 131
JO - NeuroReport
JF - NeuroReport
IS - 1
ER -