TY - JOUR
T1 - Affinity separation of messenger RNA by thermo-responsive polymer carrying oligo(dT).
AU - Mori, T.
AU - Oda, F.
AU - Umeno, D.
AU - Murata, M.
AU - Maeda, M.
PY - 1999
Y1 - 1999
N2 - The conjugate between oligo(dT)16 and thermo-responsive polymer, poly(N-isopropylacrylamide), was prepared for isolation of poly(A)+ RNA from total RNA. The hybridization reaction between the conjugate and poly(A) (average length: 320 base) was equilibrated in 10 min, and all the poly(A) (16 nmol base for 24 nmol base of conjugate) was precipitated when raising the solution temperature to 35 degrees C. The precipitate was dissolved in water, and poly(A) was dissociated from the conjugate by heating to 65 degrees C. This separation system was successfully applied to the isolation of poly(A)+ RNA from total RNA.
AB - The conjugate between oligo(dT)16 and thermo-responsive polymer, poly(N-isopropylacrylamide), was prepared for isolation of poly(A)+ RNA from total RNA. The hybridization reaction between the conjugate and poly(A) (average length: 320 base) was equilibrated in 10 min, and all the poly(A) (16 nmol base for 24 nmol base of conjugate) was precipitated when raising the solution temperature to 35 degrees C. The precipitate was dissolved in water, and poly(A) was dissociated from the conjugate by heating to 65 degrees C. This separation system was successfully applied to the isolation of poly(A)+ RNA from total RNA.
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U2 - 10.1093/nass/42.1.55
DO - 10.1093/nass/42.1.55
M3 - Article
C2 - 10780376
AN - SCOPUS:0033287653
SN - 0261-3166
SP - 55
EP - 56
JO - Nucleic acids symposium series
JF - Nucleic acids symposium series
IS - 42
ER -