抄録
To enhance the therapeutic effect of growth factors, a powerful strategy is to direct their localization to damaged sites. To treat skin wounds and myocardial infarction, we selected vascular endothelial growth factor (VEGF) carrying binding affinity to collagen. A simple conjugation of a reported collagen-binding sequence and VEGF did not increase the collagen-binding affinity, indicating that the molecular interaction between the two proteins abolished collagen binding activity. Here, we present a new molecular evolution strategy, “all-in-one” in vitro selection, in which a collagen-binding VEGF (CB-VEGF) was directly identified from a random library consisting of random and VEGF sequences. As expected, the selected CB-VEGFs exhibited high binding affinity to collagen and maintained the same growth enhancement activity for endothelial cells as unmodified VEGF in solution. Furthermore, the selected CB-VEGF enhanced angiogenesis at skin wounds and infarcted myocardium. This study demonstrates that “all-in-one” in vitro selection is a novel strategy for the design of functional proteins for regenerative medicine.
本文言語 | English |
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ページ(範囲) | 270-278 |
ページ数 | 9 |
ジャーナル | Biomaterials |
巻 | 161 |
DOI | |
出版ステータス | Published - 2018 4月 |
ASJC Scopus subject areas
- 材料力学
- セラミックおよび複合材料
- バイオエンジニアリング
- 生物理学
- 生体材料