TY - GEN
T1 - Analysis of environmental bacteria at single-cell level
AU - Hosokawa, Masahito
AU - Nishikawa, Yohei
AU - Kogawa, Masato
AU - Takeyama, Haruko
N1 - Funding Information:
This work is partly supported by Japan Science and Technology Agency – Core Research for Evolutional Science and Technology (JST-CREST) “Establishment of core technology for the preservation and regeneration of marine biodiversity and ecosystems” and Japan Science and Technology Agency – Promoting Individual Research to Nurture the Seeds of Future Innovation and Organizing Unique, Innovative Network (JST-PRESTO) “Innovative technology platform for integrated single-cell analysis”.
Publisher Copyright:
© 2017 IEEE.
PY - 2017/7/26
Y1 - 2017/7/26
N2 - Single-cell genomics has enabled the exploration of cellular diversity in environmental microbes. However, current genome sequencing techniques, which utilizes next-generation sequencing (NGS), typically require nanogram to microgram levels of input DNA sample. Since single bacterial cells contain only a few femtograms of DNA, we have to amplify their genomes to adequate amount for sequencing. We aimed to develop a novel system for precise and high throughput single-cell genomics, to elucidate environmental microbial diversity. In this study, we have developed droplet-based microfluidic system to produce the compartmentalized reaction vessels for single-cell genome sequencing.
AB - Single-cell genomics has enabled the exploration of cellular diversity in environmental microbes. However, current genome sequencing techniques, which utilizes next-generation sequencing (NGS), typically require nanogram to microgram levels of input DNA sample. Since single bacterial cells contain only a few femtograms of DNA, we have to amplify their genomes to adequate amount for sequencing. We aimed to develop a novel system for precise and high throughput single-cell genomics, to elucidate environmental microbial diversity. In this study, we have developed droplet-based microfluidic system to produce the compartmentalized reaction vessels for single-cell genome sequencing.
KW - Droplet
KW - Microfluidics
KW - NGS
KW - Single-cell genomics
UR - http://www.scopus.com/inward/record.url?scp=85029384193&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85029384193&partnerID=8YFLogxK
U2 - 10.1109/TRANSDUCERS.2017.7994129
DO - 10.1109/TRANSDUCERS.2017.7994129
M3 - Conference contribution
AN - SCOPUS:85029384193
T3 - TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems
SP - 634
EP - 637
BT - TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2017
Y2 - 18 June 2017 through 22 June 2017
ER -