Anomer selective formation of l-menthyl α-D-glucopyranoside by α-glucosidase-catalyzed reaction

Hiroyuki Nakagawa; Masaaki Yoshiyama; Susumu Shimura; Kohtaro Kirimura; Shoji Usami

doi:10.1271/bbb.60.1914

Anomer selective formation of l-menthyl α-D-glucopyranoside by α-glucosidase-catalyzed reaction

Hiroyuki Nakagawa, Masaaki Yoshiyama, Susumu Shimura, Kohtaro Kirimura, Shoji Usami^*

^*この研究の対応する著者

先進理工学部

研究成果: Article › 査読

16 被引用数 (Scopus)

抄録

L-Menthol was glucosylated by the α-glucosidase (EC 3.2.1.20) of Saccharomyces cerevisiae using maltose as glucosyl donor. When 50 mg of l-menthol and 1M maltose in 10 mM citrate–phosphate buffer (pH 7.0) were incubated for 24 h at 30°C, a menthylglucoside was selectively obtained as a product. The molar conversion yield based on supplied menthol was 4.5%. The product was identified as l-menthyl α-D-glucopyranoside (α-MenG) by ¹³C-NMR analysis.

本文言語	English
ページ（範囲）	1914-1915
ページ数	2
ジャーナル	Bioscience, Biotechnology and Biochemistry
巻	60
号	11
DOI	https://doi.org/10.1271/bbb.60.1914
出版ステータス	Published - 1996 1月

ASJC Scopus subject areas

バイオテクノロジー
分析化学
生化学
応用微生物学とバイオテクノロジー
分子生物学
有機化学

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10.1271/bbb.60.1914

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title = "Anomer selective formation of l-menthyl α-D-glucopyranoside by α-glucosidase-catalyzed reaction",

abstract = "L-Menthol was glucosylated by the α-glucosidase (EC 3.2.1.20) of Saccharomyces cerevisiae using maltose as glucosyl donor. When 50 mg of l-menthol and 1M maltose in 10 mM citrate–phosphate buffer (pH 7.0) were incubated for 24 h at 30°C, a menthylglucoside was selectively obtained as a product. The molar conversion yield based on supplied menthol was 4.5%. The product was identified as l-menthyl α-D-glucopyranoside (α-MenG) by 13C-NMR analysis.",

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author = "Hiroyuki Nakagawa and Masaaki Yoshiyama and Susumu Shimura and Kohtaro Kirimura and Shoji Usami",

year = "1996",

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doi = "10.1271/bbb.60.1914",

language = "English",

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T1 - Anomer selective formation of l-menthyl α-D-glucopyranoside by α-glucosidase-catalyzed reaction

AU - Nakagawa, Hiroyuki

AU - Yoshiyama, Masaaki

AU - Shimura, Susumu

AU - Kirimura, Kohtaro

AU - Usami, Shoji

PY - 1996/1

Y1 - 1996/1

N2 - L-Menthol was glucosylated by the α-glucosidase (EC 3.2.1.20) of Saccharomyces cerevisiae using maltose as glucosyl donor. When 50 mg of l-menthol and 1M maltose in 10 mM citrate–phosphate buffer (pH 7.0) were incubated for 24 h at 30°C, a menthylglucoside was selectively obtained as a product. The molar conversion yield based on supplied menthol was 4.5%. The product was identified as l-menthyl α-D-glucopyranoside (α-MenG) by 13C-NMR analysis.

AB - L-Menthol was glucosylated by the α-glucosidase (EC 3.2.1.20) of Saccharomyces cerevisiae using maltose as glucosyl donor. When 50 mg of l-menthol and 1M maltose in 10 mM citrate–phosphate buffer (pH 7.0) were incubated for 24 h at 30°C, a menthylglucoside was selectively obtained as a product. The molar conversion yield based on supplied menthol was 4.5%. The product was identified as l-menthyl α-D-glucopyranoside (α-MenG) by 13C-NMR analysis.

KW - Menthylglucoside

KW - Transglucosylation

KW - α-glucosidase

KW - α-glucoside formation

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Anomer selective formation of l-menthyl α-D-glucopyranoside by α-glucosidase-catalyzed reaction

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ASJC Scopus subject areas

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