Application of bacterial magnetic particles as novel DNA carriers for ballistic transformation of a marine cyanobacterium

Haruko Takeyama; Akira Yamazawa; Chikashi Nakamura; Tadashi Matsunaga

doi:10.1007/BF00638870

Application of bacterial magnetic particles as novel DNA carriers for ballistic transformation of a marine cyanobacterium

Haruko Takeyama^*, Akira Yamazawa, Chikashi Nakamura, Tadashi Matsunaga

^*この研究の対応する著者

研究成果: Article › 査読

40 被引用数 (Scopus)

抄録

Bacterial magnetite particles (BMPs) of 50 to 100nm diam were used as DNA carriers for the ballistic transformation of the marine cyanobacterium Synechococcus. BMPs were bombarded into the cyanobacterial cells at several bombardment velocities using a particle gun. Successful transformation and gene expression were confirmed by Southern hybridization and CAT assay, respectively. The BMPs were also observed in the cyanobacterial cells by transmission electron microscopy. These results suggested that BMPs can be used as carriers for introducing DNA into bacterial cells.

本文言語	English
ページ（範囲）	355-360
ページ数	6
ジャーナル	Biotechnology Techniques
巻	9
号	5
DOI	https://doi.org/10.1007/BF00638870
出版ステータス	Published - 1995 5月 1
外部発表	はい

ASJC Scopus subject areas

生化学
応用微生物学とバイオテクノロジー

UN SDG

この成果は、次の持続可能な開発目標に貢献しています

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10.1007/BF00638870

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abstract = "Bacterial magnetite particles (BMPs) of 50 to 100nm diam were used as DNA carriers for the ballistic transformation of the marine cyanobacterium Synechococcus. BMPs were bombarded into the cyanobacterial cells at several bombardment velocities using a particle gun. Successful transformation and gene expression were confirmed by Southern hybridization and CAT assay, respectively. The BMPs were also observed in the cyanobacterial cells by transmission electron microscopy. These results suggested that BMPs can be used as carriers for introducing DNA into bacterial cells.",

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AU - Takeyama, Haruko

AU - Yamazawa, Akira

AU - Nakamura, Chikashi

AU - Matsunaga, Tadashi

PY - 1995/5/1

Y1 - 1995/5/1

N2 - Bacterial magnetite particles (BMPs) of 50 to 100nm diam were used as DNA carriers for the ballistic transformation of the marine cyanobacterium Synechococcus. BMPs were bombarded into the cyanobacterial cells at several bombardment velocities using a particle gun. Successful transformation and gene expression were confirmed by Southern hybridization and CAT assay, respectively. The BMPs were also observed in the cyanobacterial cells by transmission electron microscopy. These results suggested that BMPs can be used as carriers for introducing DNA into bacterial cells.

AB - Bacterial magnetite particles (BMPs) of 50 to 100nm diam were used as DNA carriers for the ballistic transformation of the marine cyanobacterium Synechococcus. BMPs were bombarded into the cyanobacterial cells at several bombardment velocities using a particle gun. Successful transformation and gene expression were confirmed by Southern hybridization and CAT assay, respectively. The BMPs were also observed in the cyanobacterial cells by transmission electron microscopy. These results suggested that BMPs can be used as carriers for introducing DNA into bacterial cells.

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Application of bacterial magnetic particles as novel DNA carriers for ballistic transformation of a marine cyanobacterium

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ASJC Scopus subject areas

UN SDG

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