Biological determination of Ag(I) ion and arginine by using the composite film of electroinactive polypyrrole and polyion complex

Shinichi Komaba; Yusuke Fujino; Tomoka Matsuda; Tetsuya Osaka; Ikuo Satoh

doi:10.1016/S0925-4005(98)00259-7

Biological determination of Ag(I) ion and arginine by using the composite film of electroinactive polypyrrole and polyion complex

Shinichi Komaba, Yusuke Fujino, Tomoka Matsuda, Tetsuya Osaka^*, Ikuo Satoh

^*この研究の対応する著者

ナノ・ライフ創新研究機構

研究成果: Article › 査読

30 被引用数 (Scopus)

抄録

The urea biosensor based on the combination of electropolymerized insulating polypyrrole (PPy) with urease was used for the determination of Ag(I) ion and arginine. The inhibition ratio of urease with Ag(I) ion was evaluated from the urea response of the sensor. This method enabled the detection of 1.0 × 10^-7 to 1.0 × 10^-4 mol dm^-3 of Ag(I) ion. With the addition of EDTA as a masking agent for interfering heavy metal ions, the selectivity for Ag(I) ion was improved. Furthermore, arginine detection was carried out with the PPy electrode using bienzymatic combination of urease and arginase. The calibration curve for arginine was obtained in the concentration range of 1.0 × 10^-5 to 1.0 × 10^-3 mol dm^-3 with a sensitivity of 50 mV decade^-1.

本文言語	English
ページ（範囲）	78-83
ページ数	6
ジャーナル	Sensors and Actuators, B: Chemical
巻	52
号	1-2
DOI	https://doi.org/10.1016/S0925-4005(98)00259-7
出版ステータス	Published - 1998 9月 15

ASJC Scopus subject areas

電子材料、光学材料、および磁性材料
器械工学
凝縮系物理学
表面、皮膜および薄膜
金属および合金
電子工学および電気工学
材料化学

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10.1016/S0925-4005(98)00259-7

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title = "Biological determination of Ag(I) ion and arginine by using the composite film of electroinactive polypyrrole and polyion complex",

abstract = "The urea biosensor based on the combination of electropolymerized insulating polypyrrole (PPy) with urease was used for the determination of Ag(I) ion and arginine. The inhibition ratio of urease with Ag(I) ion was evaluated from the urea response of the sensor. This method enabled the detection of 1.0 × 10-7 to 1.0 × 10-4 mol dm-3 of Ag(I) ion. With the addition of EDTA as a masking agent for interfering heavy metal ions, the selectivity for Ag(I) ion was improved. Furthermore, arginine detection was carried out with the PPy electrode using bienzymatic combination of urease and arginase. The calibration curve for arginine was obtained in the concentration range of 1.0 × 10-5 to 1.0 × 10-3 mol dm-3 with a sensitivity of 50 mV decade-1.",

keywords = "Ag(I) ion, Arginine, Biosensor, Polypyrrole, Urease",

author = "Shinichi Komaba and Yusuke Fujino and Tomoka Matsuda and Tetsuya Osaka and Ikuo Satoh",

note = "Funding Information: This work was financially supported by a grant-in-aid for Scientific Research (C), the Ministry of Education, Science, Sports and Culture of Japan. ",

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AU - Komaba, Shinichi

AU - Fujino, Yusuke

AU - Matsuda, Tomoka

AU - Osaka, Tetsuya

AU - Satoh, Ikuo

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