TY - GEN
T1 - Cell-free mRNA translation in a microbiochemical reactor
AU - Hosokawa, K.
AU - Fujii, T.
AU - Nojima, T.
AU - Shoji, S.
AU - Yotsumoto, A.
AU - Endo, I.
N1 - Funding Information:
The authors would like to express their gratitude to Dr. Masafumi Yohda for his helpful advice. This work was partly supported by JSPS “Research for the Future” Program (JSPS-RFTF96100306) and the Ministry of Education, Science, Sports and Culture of Japan, Grant-in-Aid for Exploratory Research (09875203).
Publisher Copyright:
© 1997 IEEE.
PY - 1997
Y1 - 1997
N2 - A living cell is a huge network of chemical reactions in a compartmentalized microstructure. Realization of such a system using MEMS (microelectromechanical systems) technology would contribute to biological study and to development of an intelligent biochip. The authors have been focusing on protein synthesis in microreactors, because this process plays a central role in the chemical networks in living cells. This paper demonstrates that messenger RNA (mRNA)-polyuridylic acid-was translated into polypeptide-polyphenylalanine-in our primitive microreactor which was fabricated using conventional MEMS techniques: Silicon anisotropic etching and glass-silicon anodic bonding. The microreactor has a main reaction channel which is 14 millimeters long, 800 microns wide, and 20 microns deep. The amount of polyphenylalanine, which was synthesized in the channel, was determined using radioisotope assay.
AB - A living cell is a huge network of chemical reactions in a compartmentalized microstructure. Realization of such a system using MEMS (microelectromechanical systems) technology would contribute to biological study and to development of an intelligent biochip. The authors have been focusing on protein synthesis in microreactors, because this process plays a central role in the chemical networks in living cells. This paper demonstrates that messenger RNA (mRNA)-polyuridylic acid-was translated into polypeptide-polyphenylalanine-in our primitive microreactor which was fabricated using conventional MEMS techniques: Silicon anisotropic etching and glass-silicon anodic bonding. The microreactor has a main reaction channel which is 14 millimeters long, 800 microns wide, and 20 microns deep. The amount of polyphenylalanine, which was synthesized in the channel, was determined using radioisotope assay.
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U2 - 10.1109/MHS.1997.768863
DO - 10.1109/MHS.1997.768863
M3 - Conference contribution
AN - SCOPUS:0344723095
T3 - MHS 1997 - Proceedings of 1997 International Symposium on Micromechatronics and Human Science
SP - 91
EP - 95
BT - MHS 1997 - Proceedings of 1997 International Symposium on Micromechatronics and Human Science
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 8th International Symposium on Micromechatronics and Human Science, MHS 1997
Y2 - 5 October 1997 through 8 October 1997
ER -