TY - JOUR
T1 - Cell-Free Synthesis of SecYEG Translocon as the Fundamental Protein Transport Machinery
AU - Matsubayashi, Hideaki
AU - Kuruma, Yutetsu
AU - Ueda, Takuya
N1 - Publisher Copyright:
© 2015, Springer Science+Business Media Dordrecht.
Copyright:
Copyright 2016 Elsevier B.V., All rights reserved.
PY - 2014/12/1
Y1 - 2014/12/1
N2 - The cell membrane has many indispensable functions for sustaining cell alive besides a role as merely outer envelope. The most of such functions are implemented by membrane embedded proteins that are emerged through the membrane integration machinery, SecYEG translocon. Here, we synthesized SecYEG by expressing the corresponding gene in vitro to study the process of functionalization of the cell membrane.
AB - The cell membrane has many indispensable functions for sustaining cell alive besides a role as merely outer envelope. The most of such functions are implemented by membrane embedded proteins that are emerged through the membrane integration machinery, SecYEG translocon. Here, we synthesized SecYEG by expressing the corresponding gene in vitro to study the process of functionalization of the cell membrane.
KW - Cell-free protein synthesis system
KW - Liposome
KW - Membrane protein
KW - SecYEG translocon
UR - http://www.scopus.com/inward/record.url?scp=84939964635&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84939964635&partnerID=8YFLogxK
U2 - 10.1007/s11084-014-9389-y
DO - 10.1007/s11084-014-9389-y
M3 - Article
C2 - 25585802
AN - SCOPUS:84939964635
SN - 0169-6149
VL - 44
SP - 331
EP - 334
JO - Origins of Life and Evolution of Biospheres
JF - Origins of Life and Evolution of Biospheres
IS - 4
ER -