Cell rupture microfluidic device using nano needle allay for damagefree extraction of organelles

Takahiro Arakawa; Yoshitaka Shirasaki; Daisuke Yamazaki; Takashi Funatsu; Shuichi Shoji

Cell rupture microfluidic device using nano needle allay for damagefree extraction of organelles

Takahiro Arakawa, Yoshitaka Shirasaki, Daisuke Yamazaki, Takashi Funatsu, Shuichi Shoji

基幹理工学部

研究成果: Paper › 査読

1 被引用数 (Scopus)

抄録

In order to realize advanced cell function analysis, soft cell rupture device using glass nano needle arrays is developed for damage-free extraction of organelles. The cell rupture device has a simple pneumatic PDMS microvalve with nano needles fabricated on a glass substrate. By applying the pneumatic pressure, the introduced cells are pushed to the nano needles and the cell membrane is mechanically broken with small pressure. Cytoplasnic matrix (cytosol), mitochondria and lysosome trapped in the cytoskeleton are efficiently extracted within 1 sec with this device.

本文言語	English
ページ	215-217
ページ数	3
出版ステータス	Published - 2008
イベント	12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 - San Diego, CA, United States 継続期間: 2008 10月 12 → 2008 10月 16

Conference

Conference	12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008
国/地域	United States
City	San Diego, CA
Period	08/10/12 → 08/10/16

ASJC Scopus subject areas

化学工学（その他）
バイオエンジニアリング

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引用スタイル

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title = "Cell rupture microfluidic device using nano needle allay for damagefree extraction of organelles",

abstract = "In order to realize advanced cell function analysis, soft cell rupture device using glass nano needle arrays is developed for damage-free extraction of organelles. The cell rupture device has a simple pneumatic PDMS microvalve with nano needles fabricated on a glass substrate. By applying the pneumatic pressure, the introduced cells are pushed to the nano needles and the cell membrane is mechanically broken with small pressure. Cytoplasnic matrix (cytosol), mitochondria and lysosome trapped in the cytoskeleton are efficiently extracted within 1 sec with this device.",

keywords = "Cell rupture, Lysosome, Microvalve, Mitochondria, Nano needle array, Organelle",

author = "Takahiro Arakawa and Yoshitaka Shirasaki and Daisuke Yamazaki and Takashi Funatsu and Shuichi Shoji",

year = "2008",

language = "English",

pages = "215--217",

note = "12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 ; Conference date: 12-10-2008 Through 16-10-2008",

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TY - CONF

T1 - Cell rupture microfluidic device using nano needle allay for damagefree extraction of organelles

AU - Arakawa, Takahiro

AU - Shirasaki, Yoshitaka

AU - Yamazaki, Daisuke

AU - Funatsu, Takashi

AU - Shoji, Shuichi

PY - 2008

Y1 - 2008

N2 - In order to realize advanced cell function analysis, soft cell rupture device using glass nano needle arrays is developed for damage-free extraction of organelles. The cell rupture device has a simple pneumatic PDMS microvalve with nano needles fabricated on a glass substrate. By applying the pneumatic pressure, the introduced cells are pushed to the nano needles and the cell membrane is mechanically broken with small pressure. Cytoplasnic matrix (cytosol), mitochondria and lysosome trapped in the cytoskeleton are efficiently extracted within 1 sec with this device.

AB - In order to realize advanced cell function analysis, soft cell rupture device using glass nano needle arrays is developed for damage-free extraction of organelles. The cell rupture device has a simple pneumatic PDMS microvalve with nano needles fabricated on a glass substrate. By applying the pneumatic pressure, the introduced cells are pushed to the nano needles and the cell membrane is mechanically broken with small pressure. Cytoplasnic matrix (cytosol), mitochondria and lysosome trapped in the cytoskeleton are efficiently extracted within 1 sec with this device.

KW - Cell rupture

KW - Lysosome

KW - Microvalve

KW - Mitochondria

KW - Nano needle array

KW - Organelle

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M3 - Paper

AN - SCOPUS:84902482350

SP - 215

EP - 217

T2 - 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008

Y2 - 12 October 2008 through 16 October 2008

ER -

Cell rupture microfluidic device using nano needle allay for damagefree extraction of organelles

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Conference

ASJC Scopus subject areas

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