抄録
Tobacco cultivars Nicotiana tabacum cv. Samsun NN plants carrying the N gene contain a multitude of N-related genes. We cloned a few N homologues and isolated two full-length cDNAs of NL-C26 and NL-B69 genes from N. tabacum cv. Samsun NN. Nucleotide sequence analysis showed that the coding regions of NL-C26 (3,498 bp) and NL-B69 (3,510 bp) had 86 and 83% nucleotide identities with the N gene, respectively. Amino acid sequence analysis revealed that NL-C26 and NL-B69 had the Toll-interleukin-1 receptor/nucleotide-binding site/leucine-rich repeat (TIR/NBS/LRR) structure with 78 and 73% identities to N, respectively. These result indicated that NL-C26 was more similar to N than NL-B69. Tobacco mosaic virus (TMV) infection experiments suggest that NL-C26 and NL-B69 could interact with distinct avirulence (Avr) proteins of yet unidentified pathogens and function as potential HR-inducing resistance proteins similar to N.
| 本文言語 | English |
|---|---|
| ページ（範囲） | 7998-8006 |
| ページ数 | 9 |
| ジャーナル | African Journal of Biotechnology |
| 巻 | 9 |
| 号 | 47 |
| DOI | |
| 出版ステータス | Published - 2010 11月 22 |
| 外部発表 | はい |
ASJC Scopus subject areas
- バイオテクノロジー
- 応用微生物学とバイオテクノロジー
- 分子生物学
- 遺伝学
- 農業および作物学