Characterization of a Deinococcus radiodurans MazF: A UACA-specific RNA endoribonuclease

Tatsuki Miyamoto; Yuri Ota; Akiko Yokota; Tetsushi Suyama; Satoshi Tsuneda; Naohiro Noda

doi:10.1002/mbo3.501

Characterization of a Deinococcus radiodurans MazF: A UACA-specific RNA endoribonuclease

Tatsuki Miyamoto, Yuri Ota, Akiko Yokota, Tetsushi Suyama, Satoshi Tsuneda^*, Naohiro Noda

^*この研究の対応する著者

先進理工学部

研究成果: Article › 査読

9 被引用数 (Scopus)

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Microbes are known to withstand environmental stresses by using chromosomal toxin–antitoxin systems. MazEF is one of the most extensively studied toxin–antitoxin systems. In stressful environments, MazF toxins modulate translation by cleaving single-stranded RNAs in a sequence-specific fashion. Previously, a chromosomal gene located at DR0417 in Deinococcus radiodurans was predicted to code for a MazF endoribonuclease (MazF_DR ₀₄₁₇); however, its function remains unclear. In the present study, we characterized the molecular function of MazF_DR ₀₄₁₇. Analysis of MazF_DR ₀₄₁₇-cleaved RNA sites using modified massively parallel sequencing revealed a unique 4-nt motif, UACA, as a potential cleavage pattern. The activity of MazF_DR ₀₄₁₇ was also assessed in a real-time fluorometric assay, which revealed that MazF_DR ₀₄₁₇ strictly recognizes the unique tetrad UACA. This sequence specificity may allow D. radiodurans to alter its translation profile and survive under stressful conditions.

本文言語	English
論文番号	e00501
ジャーナル	MicrobiologyOpen
巻	6
号	5
DOI	https://doi.org/10.1002/mbo3.501
出版ステータス	Published - 2017 10月

ASJC Scopus subject areas

微生物学

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10.1002/mbo3.501

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title = "Characterization of a Deinococcus radiodurans MazF: A UACA-specific RNA endoribonuclease",

abstract = "Microbes are known to withstand environmental stresses by using chromosomal toxin–antitoxin systems. MazEF is one of the most extensively studied toxin–antitoxin systems. In stressful environments, MazF toxins modulate translation by cleaving single-stranded RNAs in a sequence-specific fashion. Previously, a chromosomal gene located at DR0417 in Deinococcus radiodurans was predicted to code for a MazF endoribonuclease (MazFDR 0417); however, its function remains unclear. In the present study, we characterized the molecular function of MazFDR 0417. Analysis of MazFDR 0417-cleaved RNA sites using modified massively parallel sequencing revealed a unique 4-nt motif, UACA, as a potential cleavage pattern. The activity of MazFDR 0417 was also assessed in a real-time fluorometric assay, which revealed that MazFDR 0417 strictly recognizes the unique tetrad UACA. This sequence specificity may allow D. radiodurans to alter its translation profile and survive under stressful conditions.",

keywords = "Deinococcus radiodurans, MazEF, sequence specificity, toxin–antitoxin system",

author = "Tatsuki Miyamoto and Yuri Ota and Akiko Yokota and Tetsushi Suyama and Satoshi Tsuneda and Naohiro Noda",

note = "Publisher Copyright: {\textcopyright} 2017 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.",

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doi = "10.1002/mbo3.501",

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AU - Miyamoto, Tatsuki

AU - Ota, Yuri

AU - Yokota, Akiko

AU - Suyama, Tetsushi

AU - Tsuneda, Satoshi

AU - Noda, Naohiro

PY - 2017/10

Y1 - 2017/10

N2 - Microbes are known to withstand environmental stresses by using chromosomal toxin–antitoxin systems. MazEF is one of the most extensively studied toxin–antitoxin systems. In stressful environments, MazF toxins modulate translation by cleaving single-stranded RNAs in a sequence-specific fashion. Previously, a chromosomal gene located at DR0417 in Deinococcus radiodurans was predicted to code for a MazF endoribonuclease (MazFDR 0417); however, its function remains unclear. In the present study, we characterized the molecular function of MazFDR 0417. Analysis of MazFDR 0417-cleaved RNA sites using modified massively parallel sequencing revealed a unique 4-nt motif, UACA, as a potential cleavage pattern. The activity of MazFDR 0417 was also assessed in a real-time fluorometric assay, which revealed that MazFDR 0417 strictly recognizes the unique tetrad UACA. This sequence specificity may allow D. radiodurans to alter its translation profile and survive under stressful conditions.

AB - Microbes are known to withstand environmental stresses by using chromosomal toxin–antitoxin systems. MazEF is one of the most extensively studied toxin–antitoxin systems. In stressful environments, MazF toxins modulate translation by cleaving single-stranded RNAs in a sequence-specific fashion. Previously, a chromosomal gene located at DR0417 in Deinococcus radiodurans was predicted to code for a MazF endoribonuclease (MazFDR 0417); however, its function remains unclear. In the present study, we characterized the molecular function of MazFDR 0417. Analysis of MazFDR 0417-cleaved RNA sites using modified massively parallel sequencing revealed a unique 4-nt motif, UACA, as a potential cleavage pattern. The activity of MazFDR 0417 was also assessed in a real-time fluorometric assay, which revealed that MazFDR 0417 strictly recognizes the unique tetrad UACA. This sequence specificity may allow D. radiodurans to alter its translation profile and survive under stressful conditions.

KW - Deinococcus radiodurans

KW - MazEF

KW - sequence specificity

KW - toxin–antitoxin system

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Characterization of a Deinococcus radiodurans MazF: A UACA-specific RNA endoribonuclease

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