TY - CONF
T1 - Circulatory half-life time of artificial red blood cell (hemoglobin-vesicles) and their distribution to metabolic organs
AU - Sou, Keitaro
AU - Goins, Beth
AU - Phillips, William T.
AU - Sakai, Hiromi
AU - Takeoka, Shinji
AU - Tsuchida, Eishun
PY - 2005/12/1
Y1 - 2005/12/1
N2 - Phospholipid vesicles encapsulating concentrated human hemoglobin (Hb-vesicles: HbV) have a membrane structure similar to that of red blood cells (RBC). We report the pharmacokinetics of HbV and empty vesicles (EV) that do not contain Hb, in rats and rabbits to evaluate the potential of HbV as a RBC substitute. The samples were labeled with technetium-99m (99mTc), and then intravenously infused into animals at 14 mL/kg to measure the kinetics of HbV elimination from blood and distribution to the organs. The circulation half-life times were 34.8 and 62.6 h for HbV, and 29.3 and 57.3 h for EV in rats and rabbits, respectively. At 48 h after infusion, the liver and spleen of both rats and rabbits had significant concentrations of HbV and EV. These organs would be reasonable to metabolize HbV because they have mononuclear phagocyte systems to introduce the red blood cells into metabolic process.
AB - Phospholipid vesicles encapsulating concentrated human hemoglobin (Hb-vesicles: HbV) have a membrane structure similar to that of red blood cells (RBC). We report the pharmacokinetics of HbV and empty vesicles (EV) that do not contain Hb, in rats and rabbits to evaluate the potential of HbV as a RBC substitute. The samples were labeled with technetium-99m (99mTc), and then intravenously infused into animals at 14 mL/kg to measure the kinetics of HbV elimination from blood and distribution to the organs. The circulation half-life times were 34.8 and 62.6 h for HbV, and 29.3 and 57.3 h for EV in rats and rabbits, respectively. At 48 h after infusion, the liver and spleen of both rats and rabbits had significant concentrations of HbV and EV. These organs would be reasonable to metabolize HbV because they have mononuclear phagocyte systems to introduce the red blood cells into metabolic process.
KW - Circulatory half-life time
KW - Hemoglobin
KW - Organ distribution
KW - Pharmacokinetics
KW - Phospholipid vesicles
KW - Red blood cell substitute
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M3 - Paper
AN - SCOPUS:33645650976
T2 - 54th SPSJ Annual Meeting 2005
Y2 - 25 May 2005 through 27 May 2005
ER -