Clock mutation affects circadian regulation of circulating blood cells

Katsutaka Oishi, Naoki Ohkura, Koji Kadota, Manami Kasamatsu, Kentaro Shibusawa, Juzo Matsuda, Kazuhiko Machida, Shuichi Horie, Norio Ishida*


研究成果: Article査読

72 被引用数 (Scopus)


Background: Although the number of circulating immune cells is subject to high-amplitude circadian rhythms, the underlying mechanisms are not fully understood. Methods: To determine whether intact CLOCK protein is required for the circadian changes in peripheral blood cells, we examined circulating white (WBC) and red (RBC) blood cells in homozygous Clock mutant mice. Results: Daytime increases in total WBC and lymphocytes were suppressed and slightly phase-delayed along with plasma corticosterone levels in Clock mutant mice. The peak RBC rhythm was significantly reduced and phase-advanced in the Clock mutants. Anatomical examination revealed hemoglobin-rich, swollen red spleens in Clock mutant mice, suggesting RBC accumulation. Conclusion: Our results suggest that endogenous clock-regulated circadian corticosterone secretion from the adrenal gland is involved in the effect of a Clock mutation on daily profiles of circulating WBC. However, intact CLOCK seems unnecessary for generating the rhythm of corticosterone secretion in mice. Our results also suggest that CLOCK is involved in discharge of RBC from the spleen.

ジャーナルJournal of Circadian Rhythms
出版ステータスPublished - 2006 10月 2

ASJC Scopus subject areas

  • 生理学


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