抄録
Previously, we reported that the substrate shape recognition of the Escherichia coli ribonuclease (RNase) P ribozyme depends on the concentration of magnesium ion in vitro. We additionally examined the Bacillus subtilis RNase P ribozyme and found that the B. subtilis enzyme also required high magnesium ion, above 10 mM, for cleavage of a hairpin substrate. The results of kinetic studies showed that the metal ion concentration affected both the catalysis and the affinity of the ribozymes toward a hairpin RNA substrate.
本文言語 | English |
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ページ(範囲) | 1825-1827 |
ページ数 | 3 |
ジャーナル | Bioscience, Biotechnology and Biochemistry |
巻 | 67 |
号 | 8 |
出版ステータス | Published - 2003 8月 |
外部発表 | はい |
ASJC Scopus subject areas
- 食品科学
- 応用微生物学とバイオテクノロジー
- 化学(その他)
- 生化学、遺伝学、分子生物学(全般)
- 生化学
- バイオテクノロジー
- バイオエンジニアリング