Complete genome sequence of the facultative anaerobic magnetotactic bacterium Magnetospirillum sp. strain AMB-1

Tadashi Matsunaga*, Yoshiko Okamura, Yorikane Fukuda, Aris Tri Wahyudi, Yaeko Murase, Haruko Takeyama

*この研究の対応する著者

研究成果: Article査読

185 被引用数 (Scopus)

抄録

Magnetospirillum sp. strain AMB-1 is a Gram-negative α- proteobacterium that synthesizes nano-sized magnetites, referred to as magnetosomes, aligned intracellularly in a chain. The potential of this nano-sized material is growing and will be applicable to broad research areas. It has been expected that genome analysis would elucidate the mechanism of magnetosome formation by magnetic bacteria. Here we describe the genome of Magnetospirillum sp. AMB-1 wild type, which consists of a single circular chromosome of 4 967 148 bp. For identification of genes required for magnetosome formation, transposon rnutagenesis and determination of magnetosome membrane proteins were performed. Analysis of a non-magnetic transposon mutant library focused on three unknown genes from 2752 unknown genes and three genes from 205 signal transduction genes. Partial proteome analysis of the magnetosome membrane revealed that the membrane contains numerous oxidation/reduction proteins and a signal response regulator that may function in magnetotaxis. Thus, oxidation/reduction proteins and elaborate multidomain signaling proteins were analyzed. This comprehensive genome analysis will enable resolution of the mechanisms of magnetosome formation and provide a template to determine how magnetic bacteria maintain a species-specific, nano-sized, magnetic single domain and paramagnetic morphology.

本文言語English
ページ(範囲)157-166
ページ数10
ジャーナルDNA Research
12
3
DOI
出版ステータスPublished - 2005
外部発表はい

ASJC Scopus subject areas

  • 分子生物学
  • 遺伝学

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