Conversion of protein net charge via chemical modification for highly sensitive prion detection using field effect transistor (FET) biosensor

Shofarul Wustoni, Sho Hideshima, Shigeki Kuroiwa, Takuya Nakanishi, Yasuro Mori, Tetsuya Osaka*

*この研究の対応する著者

研究成果: Article査読

5 被引用数 (Scopus)

抄録

A robust and applicable strategy of signal amplification for field effect transistor (FET) biosensor is of scientifically and technologically importance to detect wide range of target molecules, particularly for the molecules of low charge density, and to configure highly sensitive analysis. In this communication, we demonstrate a simple technique to modify the net charge of target protein based on the concept of protein conjugation for the detection of prion, which is infectious and transmissible protein in neurodegenerative disorders. The increases of prion net charge after chemical modification generate signal amplification and have remarkably enhanced the sensitivity of FET biosensor below than femtomolar (fM) level. Thus, we suggest that such strategy is promising technique for achieving a highly sensitive detection at very low concentration that is useful for early determination of protein biomarkers.

本文言語English
ページ(範囲)374-379
ページ数6
ジャーナルSensors and Actuators, B: Chemical
230
DOI
出版ステータスPublished - 2016 7月 1

ASJC Scopus subject areas

  • 電子材料、光学材料、および磁性材料
  • 器械工学
  • 凝縮系物理学
  • 表面、皮膜および薄膜
  • 金属および合金
  • 電子工学および電気工学
  • 材料化学

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