TY - JOUR
T1 - Conversion of protein net charge via chemical modification for highly sensitive prion detection using field effect transistor (FET) biosensor
AU - Wustoni, Shofarul
AU - Hideshima, Sho
AU - Kuroiwa, Shigeki
AU - Nakanishi, Takuya
AU - Mori, Yasuro
AU - Osaka, Tetsuya
N1 - Funding Information:
This work was partly supported by COI STREAM (Center of Innovation Science and Technology based Radical Innovation and Entrepreneurship Program), from the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan. Shofarul Wustoni acknowledges the graduate scholarship from Indonesia Endowment Fund for Education (LPDP), Republic of Indonesia.
Publisher Copyright:
© 2016 Elsevier B.V. All rights reserved.
PY - 2016/7/1
Y1 - 2016/7/1
N2 - A robust and applicable strategy of signal amplification for field effect transistor (FET) biosensor is of scientifically and technologically importance to detect wide range of target molecules, particularly for the molecules of low charge density, and to configure highly sensitive analysis. In this communication, we demonstrate a simple technique to modify the net charge of target protein based on the concept of protein conjugation for the detection of prion, which is infectious and transmissible protein in neurodegenerative disorders. The increases of prion net charge after chemical modification generate signal amplification and have remarkably enhanced the sensitivity of FET biosensor below than femtomolar (fM) level. Thus, we suggest that such strategy is promising technique for achieving a highly sensitive detection at very low concentration that is useful for early determination of protein biomarkers.
AB - A robust and applicable strategy of signal amplification for field effect transistor (FET) biosensor is of scientifically and technologically importance to detect wide range of target molecules, particularly for the molecules of low charge density, and to configure highly sensitive analysis. In this communication, we demonstrate a simple technique to modify the net charge of target protein based on the concept of protein conjugation for the detection of prion, which is infectious and transmissible protein in neurodegenerative disorders. The increases of prion net charge after chemical modification generate signal amplification and have remarkably enhanced the sensitivity of FET biosensor below than femtomolar (fM) level. Thus, we suggest that such strategy is promising technique for achieving a highly sensitive detection at very low concentration that is useful for early determination of protein biomarkers.
KW - Biosensor
KW - Chemical modification
KW - Field effect transistor
KW - Prion protein
KW - Protein charge
KW - Signal amplification
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U2 - 10.1016/j.snb.2016.02.078
DO - 10.1016/j.snb.2016.02.078
M3 - Article
AN - SCOPUS:84959339198
SN - 0925-4005
VL - 230
SP - 374
EP - 379
JO - Sensors and Actuators, B: Chemical
JF - Sensors and Actuators, B: Chemical
ER -