Cytoplasmic ATPase involved in ferrous ion uptake from magnetotactic bacterium Magnetospirillum magneticum AMB-1

Takeyuki Suzuki; Yoshiko Okamura; Atsushi Arakaki; Haruko Takeyama; Tadashi Matsunaga

doi:10.1016/j.febslet.2007.06.047

Cytoplasmic ATPase involved in ferrous ion uptake from magnetotactic bacterium Magnetospirillum magneticum AMB-1

Takeyuki Suzuki, Yoshiko Okamura, Atsushi Arakaki, Haruko Takeyama, Tadashi Matsunaga^*

^*この研究の対応する著者

研究成果: Article › 査読

14 被引用数 (Scopus)

抄録

A non-magnetic mutant of Magnetospirillum magneticum AMB-1 (NMA61), harboring a defective gene located in ORF4 (gene ID: amb4111) was generated by transposon mutagenesis. Biochemical characterization of the gene product of ORF4 revealed that it was localized in the cytoplasm and displayed ATPase activity. The ability of NMA61 to take up iron was severely compromised. Ferrous ion concentration in the medium decreased more with the wild-type than with NMA61, while the iron content in the cytoplasmic fraction of NMA61 was much lower than the wild-type strain. This cytoplasmic ATPase is essential for iron trafficking within M. magneticum AMB-1.

本文言語	English
ページ（範囲）	3443-3448
ページ数	6
ジャーナル	FEBS Letters
巻	581
号	18
DOI	https://doi.org/10.1016/j.febslet.2007.06.047
出版ステータス	Published - 2007 7月 24
外部発表	はい

ASJC Scopus subject areas

生物理学
構造生物学
生化学
分子生物学
遺伝学
細胞生物学

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10.1016/j.febslet.2007.06.047

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@article{edcd14f557cb4ef2825374b64ad9fe7b,

title = "Cytoplasmic ATPase involved in ferrous ion uptake from magnetotactic bacterium Magnetospirillum magneticum AMB-1",

abstract = "A non-magnetic mutant of Magnetospirillum magneticum AMB-1 (NMA61), harboring a defective gene located in ORF4 (gene ID: amb4111) was generated by transposon mutagenesis. Biochemical characterization of the gene product of ORF4 revealed that it was localized in the cytoplasm and displayed ATPase activity. The ability of NMA61 to take up iron was severely compromised. Ferrous ion concentration in the medium decreased more with the wild-type than with NMA61, while the iron content in the cytoplasmic fraction of NMA61 was much lower than the wild-type strain. This cytoplasmic ATPase is essential for iron trafficking within M. magneticum AMB-1.",

keywords = "ATPase, Cytoplasmic iron concentration, Ferrous ion transport, Magnetospirillum magneticum AMB-1, Magnetotactic bacteria",

author = "Takeyuki Suzuki and Yoshiko Okamura and Atsushi Arakaki and Haruko Takeyama and Tadashi Matsunaga",

note = "Funding Information: This work was funded in part by a Grant-in-Aid for Scientific Research (A), No. 18206084 from the Ministry of Education, Science, Sports, and Culture of Japan. ",

year = "2007",

month = jul,

day = "24",

doi = "10.1016/j.febslet.2007.06.047",

language = "English",

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T1 - Cytoplasmic ATPase involved in ferrous ion uptake from magnetotactic bacterium Magnetospirillum magneticum AMB-1

AU - Suzuki, Takeyuki

AU - Okamura, Yoshiko

AU - Arakaki, Atsushi

AU - Takeyama, Haruko

AU - Matsunaga, Tadashi

N1 - Funding Information: This work was funded in part by a Grant-in-Aid for Scientific Research (A), No. 18206084 from the Ministry of Education, Science, Sports, and Culture of Japan.

PY - 2007/7/24

Y1 - 2007/7/24

N2 - A non-magnetic mutant of Magnetospirillum magneticum AMB-1 (NMA61), harboring a defective gene located in ORF4 (gene ID: amb4111) was generated by transposon mutagenesis. Biochemical characterization of the gene product of ORF4 revealed that it was localized in the cytoplasm and displayed ATPase activity. The ability of NMA61 to take up iron was severely compromised. Ferrous ion concentration in the medium decreased more with the wild-type than with NMA61, while the iron content in the cytoplasmic fraction of NMA61 was much lower than the wild-type strain. This cytoplasmic ATPase is essential for iron trafficking within M. magneticum AMB-1.

AB - A non-magnetic mutant of Magnetospirillum magneticum AMB-1 (NMA61), harboring a defective gene located in ORF4 (gene ID: amb4111) was generated by transposon mutagenesis. Biochemical characterization of the gene product of ORF4 revealed that it was localized in the cytoplasm and displayed ATPase activity. The ability of NMA61 to take up iron was severely compromised. Ferrous ion concentration in the medium decreased more with the wild-type than with NMA61, while the iron content in the cytoplasmic fraction of NMA61 was much lower than the wild-type strain. This cytoplasmic ATPase is essential for iron trafficking within M. magneticum AMB-1.

KW - ATPase

KW - Cytoplasmic iron concentration

KW - Ferrous ion transport

KW - Magnetospirillum magneticum AMB-1

KW - Magnetotactic bacteria

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DO - 10.1016/j.febslet.2007.06.047

M3 - Article

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AN - SCOPUS:34447280419

SN - 0014-5793

VL - 581

SP - 3443

EP - 3448

JO - FEBS Letters

JF - FEBS Letters

IS - 18

ER -

Cytoplasmic ATPase involved in ferrous ion uptake from magnetotactic bacterium Magnetospirillum magneticum AMB-1

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