Deformability-Based Microfluidic Microdroplet Screening to Obtain Agarolytic Bacterial Cells

Mikihisa Muta, Wataru Kawakubo, Dong Hyun Yoon, Daiki Tanaka, Tetsushi Sekiguchi, Shuichi Shoji, Mei Ito, Yuji Hatada, Takashi Funatsu*, Ryo Iizuka*

*この研究の対応する著者

研究成果: Article査読

抄録

Environmental microorganisms possess enzymes that can digest macromolecules such as agarose into smaller molecules that can be utilized for growth. These enzymes could be valuable for the effective utilization of global resources. However, since most of the microorganisms on Earth remain uncultured, there is significant untapped enzymatic potential in nature. Therefore, it is necessary to develop innovative tools and strategies for exploring these enzymatic resources. To address this, we developed a method for screening microbial cells that secrete hydrogel-degrading enzymes using deformability-based microfluidic microdroplet sorting. In this method, microbial cells are encapsulated as single cells in water-in-oil (W/O) microdroplets with a hydrogel whose shape becomes deformable as the hydrogel is progressively degraded into smaller molecules. Screening is achieved using a microfluidic device that passively sorts the deformed W/O microdroplets. Using this method, we successfully sorted agarose-containing microdroplets, encapsulating single bacterial cells that hydrolyzed agarose. This method can be used to screen various hydrogel-degrading microbial cells.

本文言語English
ページ(範囲)16107-16114
ページ数8
ジャーナルAnalytical Chemistry
95
44
DOI
出版ステータスPublished - 2023 11月 7

ASJC Scopus subject areas

  • 分析化学

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