Design and Synthesis of d-Ala-d-Ala Silica Gel for a Binding Mode-Based Physicochemical Screening Method

Aoi Kimishima, Yuki Suzuki, Shohei Kosaka, Masako Honsho, Sota Honma, Kazunari Sakai, Hayama Tsutsumi, Yuki Inahashi, Yoshihiro Watanabe, Masato Iwatsuki*, Yukihiro Asami*, Takuji Nakashima*

*この研究の対応する著者

研究成果: Article査読

1 被引用数 (Scopus)

抄録

Vancomycin is a potent and broad-spectrum antibiotic that binds to the d-Ala-d-Ala moiety of the growing bacterial cell wall and kills bacteria. This fascinating binding model prompted us to design and synthesize d-Ala-d-Ala silica gels for the establishment of a new physicochemical (PC) screening method. In this report, we confirmed that vancomycin binds to d-Ala-d-Ala silica gel and can be eluted with MeOH containing 50 mM TFA. Finally, d-Ala-d-Ala silica gel enables to purify vancomycin from the culture broth of a vancomycin-producing strain, Amycolatopsis orientalis.

本文言語English
ページ(範囲)39035-39040
ページ数6
ジャーナルACS Omega
8
42
DOI
出版ステータスPublished - 2023 10月 24

ASJC Scopus subject areas

  • 化学一般
  • 化学工学一般

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