Destruction of organelle nuclei during spermatogenesis in Chara corallina examined by staining with DAPI and anti-DNA antibody

Ge Hong Sun, Taro Q.P. Uyeda, Tsuneyoshi Kuroiwa*

*この研究の対応する著者

研究成果: Article査読

29 被引用数 (Scopus)

抄録

The behavior of plastid and mitochondrial nuclei (synonymous with nucleoids) during spermatogenesis in Chara corallina was examined by fluorescence microscopy after staining with 4′,6-diamidino-2-phenylindole (DAPI). These organelle nuclei, which were present in internode cells and cells at the early spermatid stage, disappeared during spermatogenesis. This conclusion was confirmed by immunofluorescence microscopy using of a monoclonal anti-DNA antibody. The pattern of fluorescence obtained using the antibody coincided with that obtained by staining with DAPI. These results suggest that the disappearance of nuclei from male-derived organelles is most likely to result in maternal inheritance in Chara corallina.

本文言語English
ページ(範囲)185-188
ページ数4
ジャーナルProtoplasma
144
2-3
DOI
出版ステータスPublished - 1988 6月
外部発表はい

ASJC Scopus subject areas

  • 植物科学
  • 細胞生物学

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