Development of a dielectrophoresis-assisted surface plasmon resonance fluorescence biosensor for detection of bacteria

Chiaki Kuroda, Ryota Iizuka, Yoshimichi Ohki, Makoto Fujimaki

研究成果: Article査読

4 被引用数 (Scopus)

抄録

To detect biological substances such as bacteria speedily and accurately, a dielectrophoresis-assisted surface plasmon resonance (SPR) fluorescence biosensor is being developed. Using Escherichia coli as a target organism, an appropriate voltage frequency to collect E. coli cells on indium tin oxide quadrupole electrodes by dielectrophoresis is analyzed. Then, E. coli is stained with 4$,6-diamidino-2-phenylindole (DAPI). To clearly detect fluorescence signals from DAPI-stained E. coli cells, the sensor is optimized so that we can excite SPR on Al electrodes by illuminating 405 nm photons. As a result, the number of fluorescence signals is increased on the electrodes by the application of a low-frequency voltage. This indicates that E. coli cells with a lower permittivity than the surrounding water are collected by negative dielectrophoresis onto the electrodes where the electric field strength is lowest.

本文言語English
論文番号057001
ジャーナルJapanese journal of applied physics
57
5
DOI
出版ステータスPublished - 2018 5月

ASJC Scopus subject areas

  • 工学一般
  • 物理学および天文学一般

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