Development of a high-speed real-time polymerase chain reaction system using a circulating water-based rapid heat-exchange

Hideyuki Terazono*, Hiroyuki Takei, Akihiro Hattori, Kenji Yasuda

*この研究の対応する著者

研究成果: Article査読

13 被引用数 (Scopus)

抄録

Polymerase chain reaction (PCR) is a powerful technique to detect microorganisms, viruses, or cells by amplifying a single copy or a few copies of a fragment of a particular DNA sequence. To reduce acquisition time, it is necessary to decrease the temperature transition time between denaturation and extension. We have developed a simple rapid real-time microlitter-sample droplet PCR system accomplished by the rapid liquidbased heat-exchange of sample droplets by quick switching of two circulating hot waters of denaturation and extension, a microlitter-sized droplet and a thin-film aluminum chip. Using this system, rapid PCR amplification of a set of droplets lined up on an aluminum chip was conducted successfully as shown by the increase in fluorescence intensity, and was accomplished within 3.5 min in 40 cycles of 1 s denaturation and 3 s extension reaction, which is one magnitude faster than conventional fast PCR systems. This method allows the rapid detection of DNA fragments and has a possibility for measuring multiple samples simultaneously in a miniaturized microfluidic chip.

本文言語English
ページ(範囲)06GM051-06GM055
ジャーナルJapanese journal of applied physics
49
6 PART 2
DOI
出版ステータスPublished - 2010 6月
外部発表はい

ASJC Scopus subject areas

  • 工学一般
  • 物理学および天文学一般

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