TY - JOUR
T1 - Development of high-performance and rapid immunoassay for model food allergen lysozyme using antibody-conjugated bacterial magnetic particles and fully automated system
AU - Sato, Reiko
AU - Takeyama, Haruko
AU - Tanaka, Tsuyoshi
AU - Matsunaga, Tadashi
PY - 2001/5/23
Y1 - 2001/5/23
N2 - A high-performance and rapid chemiluminescence immunoassay for model food allergen lysozyme, one of the major allergenic components in egg white, using antibody-conjugated bacterial magnetic particles and a fully automated system was developed. This system contains a reaction station, tip rack, and an eight-tip pipettor that is able to attach and detach a strong magnet to the tip surface. The immunoreaction time was shortened to 5 min, and the assay was completed within 20 min. The lower detection limit for lysozyme was 10 ng/mL. This system can be used to perform 24 samples in 60 min within 10% coefficient of variation.
AB - A high-performance and rapid chemiluminescence immunoassay for model food allergen lysozyme, one of the major allergenic components in egg white, using antibody-conjugated bacterial magnetic particles and a fully automated system was developed. This system contains a reaction station, tip rack, and an eight-tip pipettor that is able to attach and detach a strong magnet to the tip surface. The immunoreaction time was shortened to 5 min, and the assay was completed within 20 min. The lower detection limit for lysozyme was 10 ng/mL. This system can be used to perform 24 samples in 60 min within 10% coefficient of variation.
KW - Bacterial magnetic particles
KW - Food allergen
KW - Fully automated system
KW - Lysozyme
KW - Sandwich immunoassay
UR - http://www.scopus.com/inward/record.url?scp=0035041512&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035041512&partnerID=8YFLogxK
U2 - 10.1385/ABAB:91-93:1-9:109
DO - 10.1385/ABAB:91-93:1-9:109
M3 - Article
C2 - 11963840
AN - SCOPUS:0035041512
SN - 0273-2289
VL - 91-93
SP - 109
EP - 116
JO - Applied Biochemistry and Biotechnology
JF - Applied Biochemistry and Biotechnology
ER -